アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

私たちはウェブサイトをできるだけ使いやすくするために、クッキーを使用しています。

クッキー・ポリシーでは、使用するクッキーをオプトアウトする方法について説明しています。

クッキーの設定を変更しないままでいる場合、このポリシーに同意しているとみなされます。

続ける 続ける

お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • 診断と治療のソリューション
    カスタム・ソリューション & パートナーシップ

    あなたの診断法や治療法を発展させるための、カスタム抗体開発およびコマーシャル・パートナーシップ

    私たちと共にカスタム・ソリューションを創造する

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  • ブログ
    アブカム・ブログ

    最新の情報はこちら.

    • キャンペーン
    • 製品情報
    • 技術情報

新型コロナウイルス (COVID-19)に伴う当社の対応について

  1. Link

    p4hb-antibody-rl90-ab2792.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Organelles ER
Share by email

Anti-P4HB 抗体 [RL90] (ab2792)

  • Datasheet
  • SDS
Reviews (47)Q&A (17)References (169)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

Key features and details

  • Mouse monoclonal [RL90] to P4HB
  • Suitable for: IHC-P, WB, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Rat, Hamster, Human, Pig, African green monkey
  • Isotype: IgG2a

こちらの製品もご検討ください

一次抗体
Product image
Anti-Myelin oligodendrocyte glycoprotein antibody [EP4281] (ab109746)
標識
Product image
DyLight® 405 Conjugation Kit (Fast) - Lightning-Link® (ab201798)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

製品の概要

  • 製品名

    Anti-P4HB antibody [RL90]
    P4HB 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [RL90] to P4HB
  • 由来種

    Mouse
  • アプリケーション

    適用あり: IHC-P, WB, Flow Cyt, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Rat, Hamster, Human, Pig, African green monkey
    交差が予測される動物種: Drosophila melanogaster
  • 免疫原

    Other Immunogen Type corresponding to Rat P4HB. Purified rat PDIA1/P4HB protein.
    Database link: P04785

  • ポジティブ・コントロール

    • rat liver
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    RL90
  • アイソタイプ

    IgG2a
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Proteins
    • Collagen
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Assay kits

    • P4HB Inhibitor Screening Assay Kit (ab139480)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

Our Abpromise guarantee covers the use of ab2792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 59-61 kDa (predicted molecular weight: 58 kDa). If there is no signal or signal is weak, more concentrated antibody could be used in addition to using less stringent blocking conditions (e.g., BSA instead of milk, incubating the antibody in PBST or TBST only, lower milk percentage).
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能

    This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP.
  • 配列類似性

    Belongs to the protein disulfide isomerase family.
    Contains 2 thioredoxin domains.
  • 細胞内局在

    Endoplasmic reticulum lumen. Melanosome. Cell membrane. Highly abundant. In some cell types, seems to be also secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources (Probable). Localizes near CD4-enriched regions on lymphoid cell surfaces. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Target information above from: UniProt accession P07237 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 39651 Drosophila melanogaster
    • Entrez Gene: 5034 Human
    • Entrez Gene: 18453 Mouse
    • Entrez Gene: 110255932 Pig
    • Entrez Gene: 25506 Rat
    • Omim: 176790 Human
    • SwissProt: P54399 Drosophila melanogaster
    • SwissProt: P07237 Human
    • SwissProt: P09103 Mouse
    • SwissProt: P04785 Rat
    • Unigene: 2710 Drosophila melanogaster
    • Unigene: 464336 Human
    • Unigene: 708779 Human
    • Unigene: 16660 Mouse
    • Unigene: 392196 Mouse
    • Unigene: 4234 Rat
    see all
  • 別名

    • Cellular thyroid hormone binding protein antibody
    • Cellular thyroid hormone-binding protein antibody
    • Collagen prolyl 4 hydroxylase beta antibody
    • Disulphide Isomerase antibody
    • DSI antibody
    • EC 5.3.4.1 antibody
    • Endoplasmic reticulum resident protein 59 antibody
    • ER protein 59 antibody
    • ERBA2L antibody
    • ERp59 antibody
    • GIT antibody
    • Gltathione insulin transhydrogenase antibody
    • Glutathione insulin transhydrogenase antibody
    • P4HB antibody
    • P4Hbeta antibody
    • p55 antibody
    • PDI antibody
    • PDIA1 antibody
    • PDIA1_HUMAN antibody
    • PDIR antibody
    • PHDB antibody
    • PO4DB antibody
    • PO4HB antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase) beta polypeptide (protein disulfide isomerase associated 1) antibody
    • Procollagen proline 2 oxoglutarate 4 dioxygenase beta subunit antibody
    • PROHB antibody
    • Prolyl 4 hydroxylase beta polypeptide antibody
    • Prolyl 4 hydroxylase beta subunit antibody
    • Prolyl 4 hydroxylase subunit beta antibody
    • Prolyl 4-hydroxylase subunit beta antibody
    • Protein disulfide isomerase associated 1 antibody
    • Protein disulfide isomerase, family A, member 1 antibody
    • Protein disulfide isomerase/oxidoreductase antibody
    • Protein disulfide-isomerase antibody
    • Protocollagen hydroxylase antibody
    • Thbp antibody
    • Thyroid hormone binding protein p55 antibody
    • Thyroid hormone binding protein p55 cellular antibody
    • V erb a avian erythroblastic leukemia viral oncogene homolog 2 like antibody
    see all

画像

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)

    Western blot analysis of P4HB (PDIA1) was performed by loading 25 ug of HepG2 (Lane 1) Hela (Lane 2) and NIH-3T3 (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with ab2792 at 1:1000 overnight at 4°C and washed in TBST. The membrane was then probed with a HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using a ECL Plus Western Blotting Substrate. Results show a band at approx. 57 kDa.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 550 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin at a dilution of 1:300 (1 unit/ml final concentration) for 30 minutes. Images were taken at 20X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human colon tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Overlay histogram showing HeLa cells stained with ab2792 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2792, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)This image is courtesy of an anonymous Abreview
    Anti-P4HB antibody [RL90] (ab2792) at 1/2000 dilution
    Secondary
    Donkey anti mouse IgG2a at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 57 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds

    See Abreview

  • Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Western blot - Anti-P4HB antibody [RL90] (ab2792)
    Anti-P4HB antibody [RL90] (ab2792) at 1/1000 dilution + HT1080 whole cell lysate at 20000 cells

    Secondary
    HRP-conjugated sheep anti-mouse IgG at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 57 kDa why is the actual band size different from the predicted?


    Exposure time: 20 seconds


    10% SDS-PAGE.

    Blocked with 5% milk for 1 hour at 22°C.

    Incubated with the primary for 16 hours at 4°C in PBS + 2.5% milk + 0.05% Tween20.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)This image is courtesy of an anonymous Abreview

    ab2792 staining P4HB (PDIA1) in MDA MB 231 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 1% Triton X-100 and blocked with 10% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (1/100 in BSA + 0.02% Tween 20) for 1 hour at 16°C. A DyLight® 550-conjugated goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with ab2792 (1:75) for at least 1 hour at room temperature and incubated with Dylight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin at a dilution of 1:120 (2.5units/ml final concentration) and nuclei (red) were stained with DRAQ5 at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescence analysis of P4HB (PDIA1) (green) in NIH 3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2792 (1:75) for at least 1 hour at room temperature and incubated with DyLight 488 goat anti-mouse IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin at a dilution of 1:120 (2.5 units/ml final concentration) and nuclei (blue) were stained with Hoechst at a concentration of 1ug/ml for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in p19 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in NS-1 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in HMVEC Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    Immunocytochemistry/Immunofluorescent analysis of P4HB (PDIA1) using ab2792 shows staining in A549 Cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    ab2792 positively staining dog MDCK cell ER (red) at 1/200. Staining was carried out in conjunction with goat anti mouse H and L ( Alexa 546) at 1/1000. The nuclei can be seen stained with Hoechst (blue)
    This image is courtesy of an Abreview submitted by Kun Liu on 20 September 2005. We do not have any further information relating to this image.

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)

    ab2792 positively staining formaldehyde fixed human Hek293 cells (1/200). This Ab was used in conjunction with goat anti mouse Alexa Fluor® 546 (1/1500). The nuclei has been atained with Hoechst.
    This image is courtesy of an Abreview submitted by Kun Liu on 19th September 2005. We do not have any further information relating to this image.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)
    Immunocytochemistry/ Immunofluorescence - Anti-P4HB antibody [RL90] (ab2792)This image is a courtesy of Anonymous Abreview

    ab2792 staining P4HB (PDIA1) from human HaCaT keratinocyte cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.25 % Triton ×100 and blocking with 2.5% BSA plus 1% goat serum for 1 hour at 4°C was performed. Samples were incubated with primary antibody, diluted 1/100, for 1 hour at 240C. An Alexa Fluor® 488-conjugated goat polyclonal to mouse IgG was used at dilution at 1/1000 as secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human tonsil tissue tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-P4HB antibody [RL90] (ab2792)

    Immunohistochemistry was performed on both normal and cancer biopsies of deparaffinized Human lung adenocarcinoma tissues. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing P4HB (PDIA1) ab2792 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of K562 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of NIH/3T3 cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 0.5 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

  • Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)
    Flow Cytometry - Anti-P4HB antibody [RL90] (ab2792)

    Flow cytometry analysis of P4HB (PDIA1) showing positive staining in the membrane and cytoplasm of Hela cells compared to an isotype control (blue). Cells were harvested and adjusted to a concentration of 1-5x10^6 cells/ml. Cells were then fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with ab2792 at 1 ug/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (169)

    ab2792 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2792 は 169 報の論文で使用されています。

    • Cohen CT  et al. Production and control of coagulation proteins for factor X activation in human endothelial cells and fibroblasts. Sci Rep 10:2005 (2020). PubMed: 32029851
    • Lee DS & Kim JE PDI-Mediated Reduction of Disulfide Bond on PSD95 Increases Spontaneous Seizure Activity by Regulating NR2A-PSD95 Interaction in Epileptic Rats Independent of S-Nitrosylation. Int J Mol Sci 21:N/A (2020). PubMed: 32197489
    • Mahtal N  et al. Revisiting Old Ionophore Lasalocid as a Novel Inhibitor of Multiple Toxins. Toxins (Basel) 12:N/A (2020). PubMed: 31906353
    • Huber J  et al. An atypical LIR motif within UBA5 (ubiquitin like modifier activating enzyme 5) interacts with GABARAP proteins and mediates membrane localization of UBA5. Autophagy 16:256-270 (2020). PubMed: 30990354
    • Lakshmi Narayan PK & Kajon AE Human mastadenovirus-B (HAdV-B)-specific E3-CR1ß and E3-CR1? glycoproteins interact with each other and localize at the plasma membrane of non-polarized airway epithelial cells. Virology 546:67-78 (2020). PubMed: 32452418
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 64 Abreviews or Q&A

    Western blot abreview for Anti-P4HB antibody [RL90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - other (Macrophages)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    10 µg
    Specification
    Macrophages
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 08 2018

    Western blot abreview for Anti-P4HB antibody [RL90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (heart)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    20 µg
    Specification
    heart
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 07 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (heart)
    Permeabilization
    Yes - 0.2% Triton-X
    Specification
    heart
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 03 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Hamster Cell (Chinese Hamster Ovary)
    Permeabilization
    Yes - 0,1 % TritonX-100 for 5 min @ RT
    Specification
    Chinese Hamster Ovary
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 20 2016

    IHC - Wholemount abreview for Anti-P4HB antibody [RL90]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Drosophila melanogaster Tissue (pupae wings)
    Specification
    pupae wings
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 23 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (MDA MB 231 cells)
    Permeabilization
    Yes - 1% Triton
    Specification
    MDA MB 231 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 19 2015

    Western blot abreview for Anti-P4HB antibody [RL90]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (NIH 3T3)
    Gel Running Conditions
    Reduced Denaturing (10% SDS PAGE)
    Loading amount
    50 µg
    Specification
    NIH 3T3
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    African green monkey Cell (COS-7)
    Permeabilization
    Yes - Saponin 0.05%
    Specification
    COS-7
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jan 31 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Xenopus laevis Cell (embryonic ectoderm)
    Permeabilization
    Yes - tween
    Specification
    embryonic ectoderm
    Blocking step
    BSA+milk+serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Sally Moody

    Verified customer

    投稿 May 28 2013

    Immunocytochemistry/ Immunofluorescence abreview for Anti-P4HB antibody [RL90]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeoaRG)
    Permeabilization
    Yes - 0.1% Saponin
    Specification
    HeoaRG
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 11 2013

    1-10 of 64 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 4
    • 5
    • ...
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    ブログ

    ブログを読む

    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2021 Abcam plc. All rights reserved.