Anti-p400 抗体 (ab70301)
Key features and details
- Rabbit polyclonal to p400
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-p400 antibody
p400 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p400 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region between C terminal residues 3075 and 3124 of human p400
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab70301 was affinity purified using an epitope specific to p400 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70301の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/5000. Detects a band of approximately 470 kDa (predicted molecular weight: 344 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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WB
1/2000 - 1/5000. Detects a band of approximately 470 kDa (predicted molecular weight: 344 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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機能
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 Myb-like domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 57634 Human
- Omim: 606265 Human
- SwissProt: Q96L91 Human
- Unigene: 595201 Human
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別名
- CAG repeat protein 32 antibody
- CAGH 32 antibody
- CAGH32 antibody
see all
画像
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Anti-p400 antibody (ab70301) at 0.4 µg/ml + NIH/3T3 whole cell lysate at 50 µg
Predicted band size: 344 kDa
Exposure time: 30 seconds -
All lanes : Anti-p400 antibody (ab70301) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : K-562 whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 344 kDa
Exposure time: 30 seconds -
Immunoprecipitation of ab70301 using HeLa cells.
Lane 1: rabbit anti-p400 antibody
Lane 2: ab70301 current lot
Lane 3: ab70301 previous lot
Lane4: Control IgG
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab70301 は 8 報の論文で使用されています。
- Moreno-Andrés D et al. VPS72/YL1-Mediated H2A.Z Deposition Is Required for Nuclear Reassembly after Mitosis. Cells 9:N/A (2020). PubMed: 32708675
- Moen MJ et al. An interaction network of mental disorder proteins in neural stem cells. Transl Psychiatry 7:e1082 (2017). WB . PubMed: 28375211
- Pradhan SK et al. EP400 Deposits H3.3 into Promoters and Enhancers during Gene Activation. Mol Cell 61:27-38 (2016). PubMed: 26669263
- Pérez-García V et al. Cell activation-induced phosphoinositide 3-kinase alpha/beta dimerization regulates PTEN activity. Mol Cell Biol 34:3359-73 (2014). WB ; Mouse . PubMed: 24958106
- Bellucci L et al. Activation of p21 by HDAC inhibitors requires acetylation of H2A.Z. PLoS One 8:e54102 (2013). ChIP ; Human . PubMed: 23349794
- Mattera L et al. The E1A-associated p400 protein modulates cell fate decisions by the regulation of ROS homeostasis. PLoS Genet 6: (2010). WB ; Human . PubMed: 20548951
- Kim J et al. A Myc network accounts for similarities between embryonic stem and cancer cell transcription programs. Cell 143:313-24 (2010). WB ; Mouse . PubMed: 20946988
- Young AP et al. VHL loss actuates a HIF-independent senescence programme mediated by Rb and p400. Nat Cell Biol 10:361-9 (2008). PubMed: 18297059