Anti-p400 抗体 (ab251710)
Key features and details
- Rabbit polyclonal to p400
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-p400 antibody
p400 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p400 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human p400 aa 900-1100.
Database link: Q96L91 -
ポジティブ・コントロール
- IHC-P: Human cerebral cortex, skin and testis tissue. ICC/IF: U-2 OS cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab251710の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
ターゲット情報
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機能
Component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. May be required for transcriptional activation of E2F1 and MYC target genes during cellular proliferation. The NuA4 complex ATPase and helicase activities seem to be, at least in part, contributed by the association of RUVBL1 and RUVBL2 with EP400. May regulate ZNF42 transcription activity. -
組織特異性
Ubiquitously expressed. -
配列類似性
Belongs to the SNF2/RAD54 helicase family. SWR1 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain.
Contains 1 Myb-like domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 57634 Human
- Omim: 606265 Human
- SwissProt: Q96L91 Human
- Unigene: 595201 Human
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別名
- CAG repeat protein 32 antibody
- CAGH 32 antibody
- CAGH32 antibody
see all
画像
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Paraffin-embedded human skin tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for p400 (green) using ab251710 at 4 µg/ml in ICC/IF.
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Paraffin-embedded human testis tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebral cortex tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis.
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Paraffin-embedded human liver tissue stained for p400 using ab251710 at 1/50 dilution in immunohistochemical analysis. No nuclear positivity in hepatocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab251710 は論文での使用が確認できていません。