リコンビナントRabMAb

Anti-p40 - DeltaNp63 抗体 [EPR17863-47] - BSA and Azide free (ab269956)

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Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Flow Cytometry - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
  • Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17863-47] to p40 - DeltaNp63 - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free
    p40 - DeltaNp63 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17863-47] to p40 - DeltaNp63 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HaCaT and A431 whole cell lysate. IHC-P: Human lung squamous cell carcinoma tissue. Human breast and skin tissue. Mouse and rat skin tissue. ICC/IF: HaCaT cells. Flow Cyt: HaCaT cells. IP: HaCaT whole cell lysate.

  • 特記事項

    ab269956 is the carrier-free version of ab203826. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab269956 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • バッファー

    pH: 7.2
    Constituent: PBS

  • キャリア・フリー

    はい
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR17863-47

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Human
IP
Human
アプリケーション Abreviews 特記事項
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Predicted molecular weight: 77 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 関連性

    Differentiating between lung adenocarcinoma and squamous cell carcinoma is an important distinction with therapeutic implications. Although the most frequently recommended squamous marker p63 is extremely sensitive, it suffers from low specificity due to its reactivity in a substantial proportion of lung adenocarcinomas and other tumor types, particularly lymphomas. p40 is a relatively unknown marker that recognizes ?Np63-a p63 isoform suggested to be highly specific for squamous/basal cells. p40 is equivalent to p63 in sensitivity for squamous cell carcinoma, but it is markedly superior to p63 in specificity, which eliminates a potential pitfall of misinterpreting a p63-positive adenocarcinoma or unsuspected lymphoma as squamous cell carcinoma. These findings strongly support the routine use of p40 in place of p63 for the diagnosis of pulmonary squamous cell carcinoma.

  • 参照データベース

    • 別名

      • 40 kDa Rab9 effector protein antibody
      • 8430412M01Rik antibody
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      • AV073337 antibody
      • bA65N13.1 antibody
      • C87311 antibody
      • DKFZp686P1077 antibody
      • OTTHUMP00000022126 antibody
      • OTTMUSP00000012898 antibody
      • OTTMUSP00000012899 antibody
      • OTTMUSP00000041318 antibody
      • RAB9 effector p40 antibody
      • Rab9 effector protein with Kelch motifs antibody
      • RAB9p40 antibody
      • RABEK_HUMAN antibody
      • RABEPK antibody
      • RABEPK protein antibody
      • RGD1310612 antibody
      • RP11 65N13.1 antibody
      • RP23-446N16.2 antibody
      see all

    画像

    • Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunoprecipitation - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      p40 - DeltaNp63 was immunoprecipitated from 0.35 mg HaCaT (Human skin keratinocyte) whole cell lysate 10µg with ab203826 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab203826 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: HaCaT whole cell lysate 10µg.

      Lane 2: ab203826 IP in HaCaT whole cell lysate.

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab203826 in HaCaT whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Flow Cytometry - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Flow Cytometry - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell, Left) / HaCaT (Human skin keratinocyte, Right) cells labeling p40 - DeltaNp63 with ab203826 at 1/400 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

      Negative control: HeLa. (PMID: 21631996).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunocytochemistry/ Immunofluorescence - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HaCaT (human skin keratinocyte) HeLa (human cervix adenocarcinoma epithelial cell) cells labeling p40 - DeltaNp63 with ab203826 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing strong nuclear and weak cytoplasmic staining in HaCaT cells.

      Negative control: HeLa (PMID: 21631996).

      ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the rat skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the mouse skin is observed. The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma and adenocarcinoma tissue labeling p40 - DeltaNp63 with ab203826 at a 1/1000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with hematoxylin.

      Nuclear staining on the squamous cell carcinoma (Panel A), negative staining on the adenocarcinoma (Panel B) is observed (PMID: 23486764).

      The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunohistochemical analysis of paraffin-embedded human breast tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on the basal cells of the human breast tissue is observed (PMID: 11839669; PMID: 28303349) The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

      Immunohistochemical analysis of paraffin-embedded human skin tissue labeling p40 - DeltaNp63 with ab203826 at 1/1000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on the epidermis of the human skin is observed (PMID: 11839669). The section was incubated with ab203826 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203826).

    • Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)
      Anti-p40 - DeltaNp63 antibody [EPR17863-47] - BSA and Azide free (ab269956)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

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