Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 抗体 [M138] (ab31828)
Key features and details
- Mouse monoclonal [M138] to p38 delta/MAPK13 + p38 alpha/MAPK14
- Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra), ELISA
- Knockout validated
- Reacts with: Mouse, Rat, Cow, Dog, Human, African green monkey, Syrian hamster
- Isotype: IgG1
製品の概要
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製品名
Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] -
製品の詳細
Mouse monoclonal [M138] to p38 delta/MAPK13 + p38 alpha/MAPK14 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WB, ICC/IF, Flow Cyt (Intra), ELISAmore details -
種交差性
交差種: Mouse, Rat, Cow, Dog, Human, African green monkey, Syrian hamster -
免疫原
Recombinant fragment corresponding to Rat p38 alpha/MAPK14 (C terminal). Identical to human and mouse.
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エピトープ
ab31828 recognises an epitope located in the C terminal region of p38. -
ポジティブ・コントロール
- A431 cells, Jurkat cells, HeLa cells. IHC: Human esophagus (FFPE) Flow Cyt (intra): A431 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
Purified with protein A affinity chromatography. -
ポリ/モノ
モノクローナル -
クローン名
M138 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab31828の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (14) |
1/1000. Predicted molecular weight: 41 kDa.
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ICC/IF | (2) |
1/200.
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Flow Cyt (Intra) |
1/1000.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA |
Use at an assay dependent concentration.
Peptide ELISA only. |
特記事項 |
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IHC-P
1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 41 kDa. |
ICC/IF
1/200. |
Flow Cyt (Intra)
1/1000. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. Peptide ELISA only. |
ターゲット情報
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細胞内局在
p38 alpha/MAPK14: Cytoplasm. Nucleus. -
参照データベース
- Entrez Gene: 535327 Cow
- Entrez Gene: 1432 Human
- Entrez Gene: 5603 Human
- Entrez Gene: 26415 Mouse
- Entrez Gene: 26416 Mouse
- Entrez Gene: 29513 Rat
- Entrez Gene: 81649 Rat
- Omim: 600289 Human
see all
画像
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All lanes : Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828) at 1/1000 dilution
Lane 1 :Recombinant Human p38 beta/MAPK11 protein (ab117219)
Lane 2 :Recombinant Human p38 gamma/MAPK12 protein (ab117221)
Lane 3 :Recombinant Human p38 delta/MAPK13 protein (ab113869)
Lane 4 :Recombinant Human p38 alpha/MAPK14 protein (ab82188)
Predicted band size: 41 kDa -
All lanes : Anti-p38 delta/MAPK13 + p38 alpha/MAPK14 antibody [M138] (ab31828) at 1/1000 dilution
Lane 1 : MAPK11 recombinant (ab117219)
Lane 2 : MAPK12 recombinant(ab 117221)
Lane 3 : MAPK13 recombinant (ab113869)
Lane 4 : MAPK14 (p38) recombinant (ab82188)
Lysates/proteins at 0.5 µg per lane.
Performed under reducing conditions.
Predicted band size: 41 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Green - ab31828 observed at 43 kDa.
ab31828 was shown to react with Anti-p38 antibody [M138] in Western blot. Membranes were blocked in 100% Licor before incubation with ab31828 and overnight at 4 °C at a 1 in 1000 dilution. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) secondary antibody at 1 in 20000 dilution for 1 h at room temperature before imaging.
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IHC image of ab31828 staining p38 in normal human esophagus formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31828, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: p38 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31828 observed at 40 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab31828 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab31828 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phoshpo-p38 (T180/Y182) peptide (lane 7).
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: p38 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab31828 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.This western blot image is a comparison between ab31828 and a competitor's top cited rabbit polyclonal antibody.
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Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse with ab31828. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
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Overlay histogram showing A431 cells stained with ab31828 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21828, 1:100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
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Immunocytochemical analysis of mouse embryo fibroblast cells (NIH-3T3), labelling p38 with ab31828. Sample fixed in paraformaldehyde and blocked with 1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS for 30 minutes at 22°C. Incubated with ab31828 diluted 1/500 in 1% Donkey Serum + 1% BSA + 0.1% Triton X for 1 hour at 22°C. Secondary antibody was a Donkey anti-Mouse polyclonal conjugated to Alexa Fluor® 488, diluted 1/500.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (164)
ab31828 は 164 報の論文で使用されています。
- Yang M et al. LncRNA MEG3 ameliorates NiO nanoparticles-induced pulmonary inflammatory damage via suppressing the p38 mitogen activated protein kinases pathway. Environ Toxicol 37:1058-1070 (2022). PubMed: 35006638
- Yang H et al. Fuzheng Jiedu Decoction Induces Apoptosis and Enhances Cisplatin Efficacy in Ovarian Cancer Cells In Vitro and In Vivo through Inhibiting the PI3K/AKT/mTOR/NF-κB Signaling Pathway. Biomed Res Int 2022:5739909 (2022). PubMed: 35281608
- Zheng Z et al. E. coli JM83 damages the mucosal barrier in Ednrb knockout mice to promote the development of Hirschsprung‑associated enterocolitis via activation of TLR4/p‑p38/NF‑κB signaling. Mol Med Rep 25:N/A (2022). PubMed: 35302172
- Nie B et al. Dexmedetomidine alleviates hyperalgesia in arthritis rats through inhibition of the p38MAPK signaling pathway. Immunopharmacol Immunotoxicol 44:586-593 (2022). PubMed: 35445635
- Matou-Nasri S et al. Blockade of p38 MAPK overcomes AML stem cell line KG1a resistance to 5-Fluorouridine and the impact on miRNA profiling. PLoS One 17:e0267855 (2022). PubMed: 35511922