Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK cells)
Loading amount
100 µg
Specification
MDCK cells
Gel Running Conditions
Reduced Denaturing (8% Tris-Glycine gel)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-T (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse IgG (H+L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL pico
Exposure
2 second(s)
Bands
Specific: 38 kDa Non-specific: 45 kDa
Positive control
MDCK cell lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 10 2013