Anti-p35 抗体 (ab64960)
Key features and details
- Rabbit polyclonal to p35
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-p35 antibody
p35 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p35 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide (Human) from an internal sequence
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab64960の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
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ICC/IF |
1/500 - 1/1000.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa). |
ICC/IF
1/500 - 1/1000. |
ターゲット情報
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機能
p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII. -
組織特異性
Brain and neuron specific. -
配列類似性
Belongs to the cyclin-dependent kinase 5 activator family. -
翻訳後修飾
The p35 form is proteolytically cleaved by calpain, giving rise to the p25 form. P35 has a 5 to 10 fold shorter half-life compared to p25. The conversion results in deregulation of the CDK5 kinase: p25/CDK5 kinase displays an increased and altered tau phosphorylation in comparison to the p35/CDK5 kinase in vivo.
Probably myristoylated. The Gly-2-Ala mutant is absent of the cell periphery, suggesting that a proper myristoylation signal is essential for the proper distribution of p35. -
細胞内局在
Cell membrane. In the primary cortical neurons, p35 is present in the peripheries and nerve terminals and Nucleus. Cytoplasm > perinuclear region. The conversion of p35 to p25 relocalizes the protein from the cell periphery to the cytoplasm, in nuclear and perinuclear regions. In the primary cortical neurons, p25 is primarily concentrated in the cell soma and is largely absent from neurites. - Information by UniProt
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参照データベース
- Entrez Gene: 8851 Human
- Entrez Gene: 12569 Mouse
- Entrez Gene: 116671 Rat
- Omim: 603460 Human
- SwissProt: Q15078 Human
- SwissProt: P61809 Mouse
- SwissProt: P61810 Rat
- Unigene: 500015 Human
see all -
別名
- CD5R1_HUMAN antibody
- CDK5 activator 1 antibody
- CDK5P35 antibody
see all
画像
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All lanes : Anti-p35 antibody (ab64960) at 1/500 dilution
Lane 1 : Rat brain cell extract
Lane 2 : Rat brain cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted? -
Immunofluorescence analysis of HeLa cells, using ab64960 at 1/500 dilution. Right hand panel was also treated with immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab64960 は 2 報の論文で使用されています。
- Wang H et al. Involvement of the miR-137-3p/CAPN-2 Interaction in Ischemia-Reperfusion-Induced Neuronal Apoptosis through Modulation of p35 Cleavage and Subsequent Caspase-8 Overactivation. Oxid Med Cell Longev 2020:2616871 (2020). PubMed: 33456665
- Bai X et al. CDK5 promotes renal tubulointerstitial fibrosis in diabetic nephropathy via ERK1/2/PPAR? pathway. Oncotarget 7:36510-36528 (2016). PubMed: 27145370