Anti-p27 KIP 1 抗体 [DCS-72.F6] (ab3928)

Reviews (2)Q&A (2)References (3)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [DCS-72.F6] (ab3928)

    Key features and details

    • Mouse monoclonal [DCS-72.F6] to p27 KIP 1
    • Suitable for: IHC-P
    • Reacts with: Mouse, Rat
    • Isotype: IgG1

    製品の概要

    • 製品名

      Anti-p27 KIP 1 antibody [DCS-72.F6]
      p27 KIP 1 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [DCS-72.F6] to p27 KIP 1

    • 由来種

      Mouse

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Mouse, Rat

    • 免疫原

      Recombinant full length protein corresponding to Mouse p27 KIP 1.

    • エピトープ

      Between amino acids 83-204 of p27.

    • ポジティブ･コントロール

      • Colon carcinoma.

    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    アプリケーション

    Our Abpromise guarantee covers the use of ab3928 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    ターゲット情報

    • 機能

      Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.

    • 組織特異性

      Expressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.

    • 関連疾患

      Defects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.

    • 配列類似性

      Belongs to the CDI family.

    • ドメイン

      A peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.

    • 翻訳後修飾

      Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
      Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
      Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.

    • 細胞内局在

      Nucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.
    • Information by UniProt

    • 参照データベース

      • 別名

        • AA408329 antibody
        • AI843786 antibody
        • Cdki1b antibody
        • CDKN 1B antibody
        • CDKN 4 antibody
        • CDKN1B antibody
        • CDKN4 antibody
        • CDN1B_HUMAN antibody
        • Cyclin Dependent Kinase Inhibitor 1B antibody
        • Cyclin dependent kinase inhibitor p27 antibody
        • Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
        • Cyclin-dependent kinase inhibitor 1B antibody
        • Cyclin-dependent kinase inhibitor p27 antibody
        • Cyclin-dependent kinase inhibitor p27 Kip1 antibody
        • KIP 1 antibody
        • KIP1 antibody
        • MEN1B antibody
        • MEN4 antibody
        • OTTHUMP00000195098 antibody
        • OTTHUMP00000195099 antibody
        • p27 antibody
        • p27 Kip1 antibody
        • P27-like cyclin-dependent kinase inhibitor antibody
        • p27Kip1 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [DCS-72.F6] (ab3928)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-p27 KIP 1 antibody [DCS-72.F6] (ab3928)
        ab3928 staining human colon carcinoma by IHC-P.

      参考文献 (3)

      ab3928 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3928 は 3 報の論文で使用されています。

      • Del Debbio CB  et al. Notch Signaling Activates Stem Cell Properties of Müller Glia through Transcriptional Regulation and Skp2-mediated Degradation of p27Kip1. PLoS One 11:e0152025 (2016). WB, IHC-Fr, ICC/IF ; Rat . PubMed: 27011052
      • Gómez-Herreros F  et al. TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function. Nat Genet 46:516-21 (2014). IF ; Human . PubMed: 24658003
      • Sarfraz S  et al. Modulations of cell cycle checkpoints during HCV associated disease. BMC Infect Dis 9:125 (2009). PubMed: 19664251

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-p27 KIP 1 antibody [DCS-72.F6]

       Excellent
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Liver)
      Specification
      Liver
      Fixative
      Paraformaldehyde
      Antigen retrieval step
      Heat mediated
      Permeabilization
      No
      Blocking step
      H2O2 as blocking agent for 5 minute(s) · Concentration: 0.03% · Temperature: RT°C
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      Dr. Saira Khalid

      Verified customer

      投稿 Feb 01 2010

      Question

      BATCH NUMBER -- NOT SPECIFIED -- ORDER NUMBER 158577 DESCRIPTION OF THE PROBLEM High background and non specific staining SAMPLE murine intestinal sections PRIMARY ANTIBODY overnight 4 degrees 1/100 in PBS. 3x washes in PBS DETECTION METHOD Vectorstain ABC elite kit 30 minutes at RT then 3x washes PBS and Vector DAB kit for 10-15 minutes at RT POSITIVE AND NEGATIVE CONTROLS USED Slides with no primary and no secondary both clean ANTIBODY STORAGE CONDITIONS stored at aliquots of 20ul at -20 FIXATION OF SAMPLE formalin fixed paraffin embedded ANTIGEN RETRIEVAL 10mM citrate and boiling for 20 minute sthen 40 mins to cool to room temp PERMEABILIZATION STEP incubation with pbs tween 1.5% 15mins rt BLOCKING CONDITIONS 1 hour with vector MOM bloscking buffer RT SECONDARY ANTIBODY santa cruz biotinilated goat anti rabbit with 1.5% goat serum for 1 hour at room temp. 3x washes in PBS 2 minutes HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? changed primary concentration ADDITIONAL NOTES looking for nuclear staining and finding none only in epithelial cytoplasm

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      Answer

      I'm sorry to hear you are having a problem with ab3928. If I understand your problem correctly you are seeing staining in the cytoplasm only but not in the nucleus. As you are probably aware p27 KIP 1 can be in the nucleus or cytoplasm and can also be degraded. Have you tried any other tissues? We recommend colon carcinoma as a positive control and a customer submitted a review with staining in the rat brain. Depending on cell cycle progression, the staining observed can indeed be mostly cytoplasmic. I would not have thought this result to be surprising. Do you have other evidence to suggest your staining should be only nuclear? For example, if you do Western blots on nuclear versus cytoplasmic fractions, what result do you have? Please let me know if this helps and do not hesitate to contact us for further advice.

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      Immunohistochemistry (Frozen sections) abreview for Anti-p27 KIP 1 antibody [DCS-72.F6]

       Average
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Brain (dentate gyrus))
      Specification
      Brain (dentate gyrus)
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 May 16 2006

      Question

      BATCH NUMBER 66775 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM What is the concentration of the Ab?

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      Answer

      The concentration is 100 ug/ml. Please let us know if you have any additional questions.

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