Anti-p23 抗体 (ab226295)
Key features and details
- Rabbit polyclonal to p23
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-p23 antibody
p23 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to p23 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Chicken, Cow, Orangutan -
免疫原
Synthetic peptide within Human p23 aa 110-160. The exact sequence is proprietary. (NP_006592.3).
Database link: Q15185 -
ポジティブ・コントロール
- IHC-P: Human lung cancer and ovarian carcinoma tissues. Mouse teratoma tissue. WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab226295 was affinity purified using an epitope specific to p23 immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab226295の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/1000. Predicted molecular weight: 19 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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IHC-P |
1/2000 - 1/10000.
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特記事項 |
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WB
1/2000 - 1/1000. Predicted molecular weight: 19 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
IHC-P
1/2000 - 1/10000. |
ターゲット情報
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機能
Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes. -
パスウェイ
Lipid metabolism; prostaglandin biosynthesis. -
配列類似性
Belongs to the p23/wos2 family.
Contains 1 CS domain. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 396544 Chicken
- Entrez Gene: 493638 Cow
- Entrez Gene: 10728 Human
- Entrez Gene: 100043508 Mouse
- Entrez Gene: 100048119 Mouse
- Entrez Gene: 56351 Mouse
- Entrez Gene: 100174118 Orangutan
- Entrez Gene: 362809 Rat
see all -
別名
- Co chaperone p23 antibody
- cPGES antibody
- Cytosolic prostaglandin E synthase antibody
see all
画像
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All lanes : Anti-p23 antibody (ab226295) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa
Exposure time: 30 secondsCells prepared using NETN lysis buffer.
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Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for p23 using ab226295 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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p23 was immunoprecipitated from HEK-293T (human epithelial epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226295 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226295 at 1 µg/ml.
Lane 1: ab226295 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Cells prepared using NETN lysis buffer.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for p23 using ab226295 at 1/5000 dilution in immunohistochemical analysis.
Detection: DAB staining.
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Formalin-fixed, paraffin-embedded mouse teratoma tissue stained for p23 using ab226295 at 1/1000 dilution in immunohistochemical analysis.
Detection: DAB staining.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab226295 は論文での使用が確認できていません。