Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Permeabilization
Yes - 0.3% Triton X-100 PBS
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% NDS 0.3% PBST
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Invitrogen Alexa Fluor 594 donkey anti-rat IgG (H+
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Also tried antigen retrieval using Sodium Citrate solution at both 10 & 20 mins @ 95C. Did not find any true positive signal. 10% Formalin was used for perfusion steps and spinal cord was post fixed for 4 hrs, followed by incubation in 30% sucrose-PBS. Image: dorsal horn spinal cord 20X magnification.
Abcam response
We thank this reviewer for their feedback. This antibody has not been validated to react with rat or with frozen sections (IHC-Fr).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
LAUREN DONOVAN
Verified customer
投稿 Aug 17 2020