Anti-p21 抗体 [HUGO291] (ab107099)
Key features and details
- Rat monoclonal [HUGO291] to p21
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-p21 antibody [HUGO291]
p21 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [HUGO291] to p21 -
由来種
Rat -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant full length protein corresponding to p21. HIS-GST-mp21
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ポジティブ・コントロール
- Mouse skin papilloma.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
HUGO291 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab107099の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/50. Predicted molecular weight: 18 kDa.
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IHC-P | (1) |
1/100.
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特記事項 |
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WB
1/50. Predicted molecular weight: 18 kDa. |
IHC-P
1/100. |
ターゲット情報
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機能
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. -
組織特異性
Expressed in all adult human tissues, with 5-fold lower levels observed in the brain. -
配列類似性
Belongs to the CDI family. -
ドメイン
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex. -
翻訳後修飾
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1026 Human
- Entrez Gene: 12575 Mouse
- Omim: 116899 Human
- SwissProt: P38936 Human
- SwissProt: P39689 Mouse
- Unigene: 370771 Human
- Unigene: 195663 Mouse
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別名
- CAP20 antibody
- CDK-interacting protein 1 antibody
- CDKI antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse p21WT fibrosarcoma and mouse p21KO fibrosarcoma tissue sections labeling p21 with ab107099.
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All lanes : Anti-p21 antibody [HUGO291] (ab107099) at 1/50 dilution
Lane 1 : TT243 MEFs (non-irradiated) at 18.83 µg
Lane 2 : TT243 MEFs (irradiated with 10 Gy) at 20.8 µg
Lane 3 : p21-/- KO MEFs at 60 µg
Lane 4 : P21 WT MEFs at 75 µg
Lane 5 : HEK-V5-mp21 transfected cells at 20 µg
Lane 6 : HEK-V5-mp15 transfected cells at 20 µg
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Hek-V5-mp21 transfected cells labelling p21 with ab107099. Nuclear staining was observed in Hek-V5-mp21 cytospins. Labeling with the anti-V5 mAb confirmed the efficiency of transfection. Cytospin preparation of human CD8 was used as a negative control.
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ab107099 at 1/100 staining p21 in conditional TRF1 knock out Mouse skin tissue by Immunohistochemistry.
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ab107099 at 1/100 staining p21 in Mouse skin papilloma tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab107099 は 24 報の論文で使用されています。
- Cui X et al. HMGCL-induced β-hydroxybutyrate production attenuates hepatocellular carcinoma via DPP4-mediated ferroptosis susceptibility. Hepatol Int 17:377-392 (2023). PubMed: 36508088
- Zhang G et al. NEDD4L inhibits glycolysis and proliferation of cancer cells in oral squamous cell carcinoma by inducing ENO1 ubiquitination and degradation. Cancer Biol Ther 23:243-253 (2022). PubMed: 35316145
- Gu Y et al. High TRB3 expression induces chondrocyte autophagy and senescence in osteoarthritis cartilage. Aging (Albany NY) 14:5366-5375 (2022). PubMed: 35776529
- Chung EJ et al. IGF-1 Receptor Signaling Regulates Type II Pneumocyte Senescence and Resulting Macrophage Polarization in Lung Fibrosis. Int J Radiat Oncol Biol Phys 110:526-538 (2021). PubMed: 33385497
- Viais R et al. Augmin deficiency in neural stem cells causes p53-dependent apoptosis and aborts brain development. Elife 10:N/A (2021). PubMed: 34427181