Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testes and lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization
No
Specification
testes and lung
Blocking step
Protein Block as blocking agent for 10 minute(s) · Concentration: 100% · Temperature: 21°C
Fixative
NBF
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: Dako Antibody Diluent
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Envision Rabbit HRP Polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Also tried HIER with Tris-EDTA, pH9, Pepsin, Proteinase K, Citrate HIER+ Pepsin and Citrate HIER + Proteinase K for retrieval. Assay buffer contained Tween 20. ALl conditions tested resulted in no staining. We do not think that this antibody is suitable for IHC-P in mouse samples.
Abcam response
We would like to thank the reviewer for submitting this AbReview for this product. As the product had not yet been tested in this application we were unsure what the result might be and we appreciate the time and effort that the reviewer has put in to testing this product in this application. We encourage all researchers to contact us should they wish to test any of our products in untested species or applications, as these may qualify for our AbTrial testing program.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jul 15 2021