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RabMAb

Anti-p130 (phospho T986) 抗体 [EPR2389(2)] (ab211928)

製品の概要

  • 製品名

    Anti-p130 (phospho T986) antibody [EPR2389(2)]
    p130 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR2389(2)] to p130 (phospho T986)
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, IP, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p130 aa 950-1050 (phospho T986). The exact sequence is proprietary.
    Database link: Q08999

  • ポジティブ・コントロール

    • WB: Jurkat and NIH/3T3 whole cell lysates. Flow Cyt: Jurkat cells. IP: NIH/3T3 whole cell lysate.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab211928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 128 kDa (predicted molecular weight: 128 kDa).
IP 1/100.
Flow Cyt 1/200.

ターゲット情報

  • 機能

    Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation, associates preferentially with E2F5. Binds to cyclins A and E. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. May act as a tumor suppressor.
  • 配列類似性

    Belongs to the retinoblastoma protein (RB) family.
  • 発生段階

    G0-restricted expression.
  • 翻訳後修飾

    During G0 and early G1 phase of the cell cycle, phosphorylated on Ser-639 and on 5 sites within the domain B. Phosphorylation on Ser-672 in G1 leads to its ubiquitin-dependent proteolysis.
  • 細胞内局在

    Nucleus.
  • Information by UniProt
  • 参照データベース

  • 別名

    • 130 kDa retinoblastoma associated protein antibody
    • 130 kDa retinoblastoma-associated protein antibody
    • IB4 antibody
    • p130 antibody
    • PPAR-alpha-interacting complex protein 128 antibody
    • PRB2 antibody
    • Rb2 antibody
    • Rbl2 antibody
    • RBL2_HUMAN antibody
    • RBR 2 antibody
    • RBR-2 antibody
    • RBR2 antibody
    • Retinoblastoma like 2 antibody
    • Retinoblastoma-like protein 2 antibody
    • Retinoblastoma-related protein 2 antibody
    • RIC128 antibody
    see all

画像

  • All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate treated with phosphatase on the membrane

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 15 seconds


    Blocking/Dilution buffer: 2% BSA.

  • All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate treated with phosphatase on the membrane

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-p130 (phospho T986) antibody [EPR2389(2)] (ab211928) at 1/1000 dilution

    Lane 1 : Untreated Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with single phospho peptide
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with non phospho peptide

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 128 kDa
    Observed band size: 128 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling p130 (phospho T986) with ab211928 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • p130 (phospho T986) was immunoprecipitated from 0.35 mg of NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate with ab211928 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab211928 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: NIH/3T3 whole cell lysate 10µg (Input).

    Lane 2: ab211928 IP in NIH/3T3 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211928 in NIH/3T3 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

     

参考文献

ab211928 has not yet been referenced specifically in any publications.

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