Anti-p115-RhoGEF 抗体 [JH-1] (ab243248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Armenian hamster monoclonal [JH-1] to p115-RhoGEF
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, ICC
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-p115-RhoGEF antibody [JH-1]
p115-RhoGEF 一次抗体 製品一覧 -
製品の詳細
Armenian hamster monoclonal [JH-1] to p115-RhoGEF -
由来種
Armenian hamster -
アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, ICCmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment within Mouse p115-RhoGEF. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q61210 -
ポジティブ・コントロール
- WB: A20, EL4, PC-12 and C6 whole cell lysates. ICC: A20, Neuro2a and EL4 cells. Flow Cyt (intra): EL4 cells.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
JH-1 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243248の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/40.
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WB |
1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa).
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ICC/IF |
1/50.
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ICC |
1/50.
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特記事項 |
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Flow Cyt (Intra)
1/40. |
WB
1/1000. Detects a band of approximately 115 kDa (predicted molecular weight: 102 kDa). |
ICC/IF
1/50. |
ICC
1/50. |
ターゲット情報
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機能
Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation. -
組織特異性
Ubiquitously expressed. -
配列類似性
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 RGSL (RGS-like) domain. -
ドメイン
The RGSL domain, also known as rgRGS domain, is necessary but not sufficient for GAP activity.
The DH domain is involved in interaction with CCPG1. -
翻訳後修飾
Phosphorylated by PKCA. Angiotensin-2 induced Tyr-738 phosphorylation is mediated by JAK2. -
細胞内局在
Cytoplasm. Membrane. Translocated to the membrane by activated GNA13 or LPA stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 16801 Mouse
- Entrez Gene: 60323 Rat
- SwissProt: Q61210 Mouse
- SwissProt: Q9Z1I6 Rat
- Unigene: 3181 Mouse
- Unigene: 64481 Rat
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別名
- 115 kD protein antibody
- 115 kDa guanine nucleotide exchange factor antibody
- ARHG1_HUMAN antibody
see all
画像
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ICC/IF image of ab243248 stained Neuro2a cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab243248, 1.6µg/ml) overnight at +4°C. The secondary antibody (orange) was ab175716 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 568) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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ICC/IF image of ab243248 stained Neuro2a cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab243248, 1.6µg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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All lanes : Anti-p115-RhoGEF antibody [JH-1] (ab243248) at 1/1000 dilution
Lane 1 : A20 (mouse reticulum sarcoma B lymphocyte), whole cell lysate
Lane 2 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 4 : C6 (rat glial tumor glial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit Anti-Armenian hamster IgG H&L (HRP) (ab5745) at 1/5000 dilution
Predicted band size: 102 kDa
Observed band size: 115 kDa why is the actual band size different from the predicted?The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11384980).
Lysates should be made freshly and used in WB immediately.Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lanes 1/2:3 seconds; Lane 3:15 seconds; Lane 4:37 seconds.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A20 (mouse reticulum sarcoma B lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in A20 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized EL-4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/40 dilution (1µg) (Red) compared with an Armenian hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/2000 dilution was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling p115-RhoGEF with ab243248 at 1/50 dilution, followed by ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in EL4 cells. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/1000 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab243248 at a 1/50 dilution followed by ab150080 at a 1/1000 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab173003 at a 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab243248 は論文での使用が確認できていません。