Anti-P cadherin 抗体 - N-terminal (ab190076)
Key features and details
- Rabbit polyclonal to P cadherin - N-terminal
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-P cadherin antibody - N-terminal
P cadherin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to P cadherin - N-terminal -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human P cadherin (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). NP_001784.2.
Database link: P22223 -
ポジティブ・コントロール
- WB: NIH 3T3 cell lysate. IHC-P: Human pancreatic duct and prostate carcinoma tissue. Flow Cyt (Intra): NCI-H292 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190076の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 91 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/1000. Predicted molecular weight: 91 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. -
組織特異性
Expressed in some normal epithelial tissues and in some carcinoma cell lines. -
関連疾患
Defects in CDH3 are the cause of hypotrichosis with juvenile macular dystrophy (HJMD) [MIM:601553]. HJMD is a rare autosomal recessive disorder characterized by early hair loss heralding severe degenerative changes of the retinal macula and culminating in blindness during the second to third decade of life.
Defects in CDH3 are the cause of ectodermal dysplasia with ectrodactyly and macular dystrophy (EEM) [MIM:225280]; also known as EEM syndrome, Albrectsen-Svendsen syndrome or Ohdo-Hirayama-Terawaki syndrome. Ectodermal dysplasia defines a heterogeneous group of disorders due to abnormal development of two or more ectodermal structures. EEM is an autosomal recessive condition characterized by features of ectodermal dysplasia such as sparse eyebrows and scalp hair, and selective tooth agenesis associated with macular dystrophy and ectrodactyly. -
配列類似性
Contains 5 cadherin domains. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1001 Human
- Entrez Gene: 12560 Mouse
- Omim: 114021 Human
- SwissProt: P22223 Human
- SwissProt: P10287 Mouse
- Unigene: 191842 Human
- Unigene: 4658 Mouse
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別名
- CADH3_HUMAN antibody
- Cadherin 3 antibody
- Cadherin 3 precursor antibody
see all
画像
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Anti-P cadherin antibody - N-terminal (ab190076) at 1/1000 dilution + NIH 3T3 cell lysate at 35 µg
Predicted band size: 91 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreatic duct tissue labeling P cadherin with ab190076 at 10 µg/ml.
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Intracellular flow cytometric analysis of NCI-H292 cells labeling P Cadherin with ab190076 at 1/10 dilution (bottom histogram) compared to negative control cells (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling P cadherin with ab190076 at 10 µg/ml, followed by peroxidase-conjugated secondary antibody and DAB staining.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab190076 は 2 報の論文で使用されています。
- Cai X et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
- Mammadova A et al. Deregulated Adhesion Program in Palatal Keratinocytes of Orofacial Cleft Patients. Genes (Basel) 10:N/A (2019). PubMed: 31652793