製品の概要
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製品名Anti-Oxytocin-neurophysin 1 antibody
Oxytocin-neurophysin 1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Oxytocin-neurophysin 1
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由来種Rabbit
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特異性Less than 1% reactivity to vasopressin.
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アプリケーション適用あり: IHC-FoFr, ICC/IF, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Sheep, Rabbit, Guinea pig, Human, Pig
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免疫原
Other Immunogen Type corresponding to Oxytocin-neurophysin 1. Immunogen: oxytocin-thyroglobulin
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ポジティブ･コントロール
- ICC/IF: HepG2 cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーConstituent: Whole serum
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 20043984 | |
| ICC/IF | 1/200. | |
| IHC-P | 1/10000. |
ターゲット情報
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関連性Neurophysin 1 specifically binds oxytocin. Oxytocin causes contraction of the smooth muscle of the uterus and of the mammary gland. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Oxytocin contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions.
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細胞内局在Secreted
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参照データベース
- Entrez Gene: 5020 Human
- Entrez Gene: 18429 Mouse
- Entrez Gene: 25504 Rat
- Omim: 167050 Human
- SwissProt: P01178 Human
- SwissProt: P35454 Mouse
- SwissProt: P01177 Pig
- SwissProt: P13389 Sheep
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別名
- MGC126890 antibody
- MGC126892 antibody
- Neurophysin 1 antibody
see all
画像
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Oxytocin-neurophysin 1 antibody (ab2078)Image from Ettrup KS et al, J Chem Neuroanat. 2010 May;39(3):151-65. Epub 2009 Dec 28, Fig 2, doi:10.1016/j.jchemneu.2009.12.004.Minipigs were deeply anesthetized with a combination of midazolam and ketamine, prior to transcardial perfusion with phosphate buffered 4% paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
Immunohistochemistry was performed using the avidin-biotin method. Accordingly, free-floating sections were first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with avidin (0.1%) and -
Immunocytochemistry/ Immunofluorescence - Anti-Oxytocin-neurophysin 1 antibody (ab2078)ab2078 stained HepG2 (human liver hepatocellular carcinoma cell line) cells. The cells were 4% formaldehyde fixed (10 minutes) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2078 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.
参考文献
This product has been referenced in:
- Mohan S et al. Oxytocin is present in islets and plays a role in beta-cell function and survival. Peptides 100:260-268 (2018). IF . Read more (PubMed: 29274352) »
- Calhan OY & Seyrantepe V Mice with Catalytically Inactive Cathepsin A Display Neurobehavioral Alterations. Behav Neurol 2017:4261873 (2017). IHC-Fr ; Mouse . Read more (PubMed: 28133419) »
レビューと Q&A
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