Application
Western blot
Sample
Mouse Cell lysate - other (Mice brain, and cell line mitochondria)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Mice brain, and cell line mitochondria
Blocking step
SEA BLOCK Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SEA BLOCK Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/30000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IRDye 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared LiCor scanner
Negative control
5 - Non-Synaptosomal mice brain mitochondria
6 - Synaptosomal mice brain mitochondria
7 - STHdh-Q7/Q7 mitochondria
8 - STHdh-Q111/Q111 mitochondria
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 52 kDa Non-specific: 70, 25, 20 kDa
Positive control
1 - HEK293 Whole cell lysate
2 - Mitochondria isolated from HEK293 cells
3 - Non-Synaptosomal human brain mitochondria
4 - Synaptosomal human brain mitochondria
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Svitlana Yablonska
Verified customer
投稿 Aug 24 2015