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Anti-Osteoprotegerin 抗体 [bony-1] (ab18051)

Submit a review Q&A (1)
Flow Cytometry - Anti-Osteoprotegerin antibody [bony-1] (ab18051)

    Key features and details

    • Rat monoclonal [bony-1] to Osteoprotegerin
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-Osteoprotegerin antibody [bony-1]
      Osteoprotegerin 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [bony-1] to Osteoprotegerin

    • 由来種

      Rat

    • 特異性

      Recognizes human OPG

    • アプリケーション

      適用あり: Flow Cytmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant fragment, corresponding to amino acids 22-202 of Human Osteoprotegerin.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab18051の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Flow Cyt
    1/1000.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
    特記事項
    Flow Cyt
    1/1000.

    ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

    ターゲット情報

    • 機能

      Acts as decoy receptor for RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local RANKL/OPG ratio. May also play a role in preventing arterial calcification. May act as decoy receptor for TRAIL and protect against apoptosis. TRAIL binding blocks the inhibition of osteoclastogenesis.

    • 組織特異性

      Highly expressed in adult lung, heart, kidney, liver, spleen, thymus, prostate, ovary, small intestine, thyroid, lymph node, trachea, adrenal gland, testis, and bone marrow. Detected at very low levels in brain, placenta and skeletal muscle. Highly expressed in fetal kidney, liver and lung.

    • 関連疾患

      Defects in TNFRSF11B are the cause of juvenile Paget disease (JPD) [MIM:239000]; also known as hyperostosis corticalis deformans juvenilis or hereditary hyperphosphatasia or chronic congenital idiopathic hyperphosphatasia. JPD is a rare autosomal recessive osteopathy that presents in infancy or early childhood. The disorder is characterized by rapidly remodeling woven bone, osteopenia, debilitating fractures, and deformities due to a markedly accelerated rate of bone remodeling throughout the skeleton. Approximately 40 cases of JPD have been reported worldwide. Unless it is treated with drugs that block osteoclast-mediated skeletal resorption, the disease can be fatal.

    • 配列類似性

      Contains 2 death domains.
      Contains 4 TNFR-Cys repeats.

    • 翻訳後修飾

      N-glycosylated. Contains sialic acid residues.
      The N-terminus is blocked.

    • 細胞内局在

      Secreted.
    • Information by UniProt

    • 参照データベース

      • 別名

        • MGC29565 antibody
        • OCIF antibody
        • OPG antibody
        • Osteoclastogenesis inhibitory factor antibody
        • Osteoprotegerin antibody
        • PDB5 antibody
        • TNF receptor superfamily member 11b antibody
        • TNFRSF 11B antibody
        • TNFRSF11B antibody
        • TR 1 antibody
        • TR1 antibody
        • TR11B_HUMAN antibody
        • Tumor necrosis factor receptor superfamily member 11B antibody
        see all

      画像

      • Flow Cytometry - Anti-Osteoprotegerin antibody [bony-1] (ab18051)
        Flow Cytometry - Anti-Osteoprotegerin antibody [bony-1] (ab18051)

        FACS analysis of cells with ab18051.
        HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of the TNFR homology domain of human OPG (thick line). Cells were incubated on ice for 30 min in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide)
        containing 1µg/ml of ab18051. After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by Flow Cytometry.

      参考文献 (0)

      ab18051 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab18051 は論文での使用が確認できていません。

      レビューと Q&A

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      レビューを送る 質問を送る

      Question

      Dear Sir Madam, I would like to ask whether the following antibodies are known to be neutralising as this is an important requirement for my particular assay. I couldn't find this information on the website: Rat Monoclonal to Human Osteoprotegerin - ab18051 Rabbit Polyclonal to human Osteoprotegerin - ab 9986 Kind Regards

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      Answer

      Thank you for contacting us. Unfortunately both anti-Osteoprotegerin antibody ab18051 and ab9986 have not as yet been assessed for their neutralising activity to our knowledge. This does not mean that these will not work as a neutralising antibodies. However, the only anti-Osteoprotegerin antibody which has been assessed for this property is goat polyclonal ab10962, but this is directed against the mouse protein and only shows a 25% cross reactivity with the human protein. If you would like to purchase ab18051 or ab9986 for their use in neutralising assays we can offer you a testing discount. This offer means that if you test the antibody for its neutralising activity with human Osteoprotegerin and let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalog. More information on this offer can be found here:   www.abcam.com/collaborationdiscount If you would be interested in this offer please let me know as the discount code needs to be issued prior to purchase of the antibody. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.  

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