Anti-Osteopontin 抗体 (ab8448)
Key features and details
- Rabbit polyclonal to Osteopontin
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-Osteopontin antibody
Osteopontin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Osteopontin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human, Pig -
免疫原
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.1% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab8448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/100 - 1/300. | |
| ICC/IF | 1/1000. | |
| WB | 1/1000. at this dilution, the antibody will strongly detect approximately 250 ng of OPN protein on a blot. |
ターゲット情報
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機能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
組織特異性
Bone. Found in plasma. -
配列類似性
Belongs to the osteopontin family. -
翻訳後修飾
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6696 Human
- Entrez Gene: 20750 Mouse
- Entrez Gene: 397087 Pig
- Entrez Gene: 25353 Rat
- Omim: 166490 Human
- SwissProt: P10451 Human
- SwissProt: P10923 Mouse
- SwissProt: P14287 Pig
see all -
別名
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)Joshi, S. et al PLoS One. 2017 Nov 1;12(11):e0185009. doi: 10.1371/journal.pone.0185009. eCollection 2017. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Immuno-histological staining of EG treated rat kidney
Formalin-fixed, paraffin-embedded rat kidney tissue stained for Osteopontin using ab8448 at 1/100 dilution in immunohistochemical analysis.
A) Control B) on Day 14 showing light staining of the tubular epithelial cells, shown by black arrows and C) on Day 28 showing strong staining of renal tubular epithelial cells as well as tubular contents (Black arrows), Magnification, X 40, Scale bar 100μm.
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Western blot - Anti-Osteopontin antibody (ab8448)All lanes : Anti-Osteopontin antibody (ab8448) at 1/1000 dilution
Lane 2 : Human Osteopontin
Lane 3 : MMP-cleaved Human Osteopontin
Lysates/proteins at 0.25 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/10000 dilution
The osteopontin antibody (ab8448) is used at 1:1000 dilution on a blot with 250ng human osteopontin (lane 2)and MMP-cleaved osteopontin (lane 3 -
Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (ab8448)ICC/IF image of ab8448 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8448, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.
Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)Image is courtesy of an Abreview submitted by Helder Fonsecaab8448 staining Osteopontin in Mouse femur tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 1 hour at 35°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50) for 14 hours at 4°C. An Alkaline Phosphatase-conjugated Goat anti-rabbit IgG F(ab')2 polyclonal (1/100) was used as the secondary antibody.
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Other - Anti-Osteopontin antibody (ab8448)This image is courtesy of Agnihotri et al, JBC 2001OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.
プロトコール
参考文献 (216)
ab8448 は 216 報の論文で使用されています。
- Li L et al. XIST/miR-376c-5p/OPN axis modulates the influence of proinflammatory M1 macrophages on osteoarthritis chondrocyte apoptosis. J Cell Physiol 235:281-293 (2020). PubMed: 31215024
- Geng Y et al. Overexpression of mechanical sensitive miR-337-3p alleviates ectopic ossification in rat tendinopathy model via targeting IRS1 and Nox4 of tendon-derived stem cells. J Mol Cell Biol 12:305-317 (2020). PubMed: 31065679
- Li P et al. Loss of PARP-1 attenuates diabetic arteriosclerotic calcification via Stat1/Runx2 axis. Cell Death Dis 11:22 (2020). PubMed: 31924749
- Marycz K et al. Iron oxides nanoparticles (IOs) exposed to magnetic field promote expression of osteogenic markers in osteoblasts through integrin alpha-3 (INTa-3) activation, inhibits osteoclasts activity and exerts anti-inflammatory action. J Nanobiotechnology 18:33 (2020). PubMed: 32070362
- Gadalla D & Goldstein AS Improving the Osteogenicity of PCL Fiber Substrates by Surface-Immobilization of Bone Morphogenic Protein-2. Ann Biomed Eng 48:1006-1015 (2020). PubMed: 31115719
