Anti-ORP150 抗体 [EP5891] (ab134944)
Key features and details
- Rabbit monoclonal [EP5891] to ORP150
- Suitable for: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ORP150 antibody [EP5891]
ORP150 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP5891] to ORP150 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IP, ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human ORP150 aa 950-1050 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Human colon and liver tissues; MCF7 cells; 293T, HeLa, HepG2, MCF7 and SH-SY5Y cell lysates IP: HeLa whole cell lysate.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20ºC. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP5891 -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab134944の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
1/10 - 1/100.
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ICC/IF |
1/250 - 1/500.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 111 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
1/10 - 1/100. |
ICC/IF
1/250 - 1/500. |
WB
1/1000 - 1/10000. Predicted molecular weight: 111 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. -
組織特異性
Highly expressed in tissues that contain well-developed endoplasmic reticulum and synthesize large amounts of secretory proteins. Highly expressed in liver and pancreas and lower expression in brain and kidney. Also expressed in macrophages within aortic atherosclerotic plaques, and in breast cancers. -
配列類似性
Belongs to the heat shock protein 70 family. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 10525 Human
- Omim: 601746 Human
- SwissProt: Q9Y4L1 Human
- Unigene: 277704 Human
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別名
- 150 kDa oxygen regulated protein antibody
- 150 kDa oxygen-regulated protein antibody
- 170 kDa glucose regulated protein antibody
see all
画像
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All lanes : Anti-ORP150 antibody [EP5891] (ab134944) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 111 kDa -
Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ORP150 with unpurified ab134944 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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purifiedab134944 at 1/50 dilution (2µg) immunoprecipitating ORP150 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab134944 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab134944 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/2000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 150 kDa -
Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.
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Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab134944 は 5 報の論文で使用されています。
- Liu J & Wang Y Long non-coding RNA KCNQ1OT1 facilitates the progression of cervical cancer and tumor growth through modulating miR-296-5p/HYOU1 axis. Bioengineered 12:8753-8767 (2021). PubMed: 34704918
- Kuljittichanok D et al. Effect of Derris scandens extract on a human hepatocellular carcinoma cell line. Oncol Lett 16:1943-1952 (2018). PubMed: 30034552
- Abu El-Asrar AM et al. Association of 150-kDa oxygen-regulated protein with vascular endothelial growth factor in proliferative diabetic retinopathy. Acta Ophthalmol 96:e460-e467 (2018). PubMed: 29098793
- Giffin L et al. Modulation of Kaposi's sarcoma-associated herpesvirus interleukin-6 function by hypoxia-upregulated protein 1. J Virol 88:9429-41 (2014). PubMed: 24920810
- Cui GQ et al. Proteomic analysis of meningiomas. Acta Neurol Belg 114:187-94 (2014). WB ; Human . PubMed: 24085542