Anti-Orexin A 抗体 (ab6214)
Key features and details
- Rabbit polyclonal to Orexin A
- Suitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-P
- Reacts with: Mouse, Rat, Cow
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Orexin A antibody
Orexin A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Orexin A -
由来種
Rabbit -
特異性
Immunohistochemical analysis of rat brain indicates a good specificity for this serum. -
アプリケーション
適用あり: ELISA, IHC-Fr, IHC-FoFr, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Cow -
免疫原
Synthetic peptide corresponding to Cow Orexin A aa 1-100 conjugated to keyhole limpet haemocyanin (Glutaraldehyde).
Database link: P56717 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7 -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6214の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 16052497
Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4 degree C. |
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| IHC-P | (2) |
Use at an assay dependent concentration.
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| 特記事項 |
|---|
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ELISA
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 16052497 Following perfusion fixation with 4% PFA, cryoprotect the tissue in 20% sucrose overnight. Cut on cryostat sections for free floating IHC. Incubate primary antibody for 48 hrs at 4 degree C. |
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IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Orexin A (hypocretin 1) a 33 amino acid peptide and orexin B (hypocretin 2), a 28 amino acid peptide, are both derived from a common 130 amino acid precursor, prepro orexin. Orexin A and Orexin B stimulate food consumption when administered intracerebroventricularly to rats. Orexin gene expression in the brain is highly restricted to distinct populations of neurons located in specific hypothalamic regions, including the lateral hypothalamic area (LHA), a region implicated in feeding behaviour. Orexin A and orexin B bind to and activate two closely related G protein coupled receptors (GPCRs), termed Orexin 1 (OX1) and Orexin 2 (OX2) receptors. Dysfunction of the orexin peptide system has been linked to narcolepsy. -
細胞内局在
Rough endoplasmic reticulum. Cytoplasmic vesicle. Cell junction, synapse. Note: Associated with perikaryal rough endoplasmic reticulum as well as cytoplasmic large granular vesicles at synapses. -
参照データベース
- Entrez Gene: 15171 Mouse
- Entrez Gene: 25723 Rat
- SwissProt: O55241 Mouse
- SwissProt: O55232 Rat
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別名
- HCRT antibody
- HCRT1 antibody
- HCRT2 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)
Immunohistochemical staining of orexin A in rat lateral hypothalamus useing Anti-Orexin A antibody (ab6214) at 1:50000 dilution. The primary antibody was detected with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Orexin A antibody (ab6214)Immunohistochemical staining of orexin A in rat zona incerta neurons using Rabbit antibody to Orexin A (ab6214) at a dilution of 1 in 1000.
データシートおよび資料
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Datasheet download
参考文献 (7)
ab6214 は 7 報の論文で使用されています。
- Zhang B et al. Hypothalamic orexin and mechanistic target of rapamycin activation mediate sleep dysfunction in a mouse model of tuberous sclerosis complex. Neurobiol Dis 134:104615 (2020). PubMed: 31605778
- Wang Y et al. Orexins alleviate motor deficits via increasing firing activity of pallidal neurons in a mouse model of Parkinson's disease. Am J Physiol Cell Physiol 317:C800-C812 (2019). PubMed: 31365289
- Kakava-Georgiadou N et al. Considerations related to the use of short neuropeptide promoters in viral vectors targeting hypothalamic neurons. Sci Rep 9:11146 (2019). PubMed: 31366942
- Franco-Pérez J et al. Chronic Consumption of Fructose Induces Behavioral Alterations by Increasing Orexin and Dopamine Levels in the Rat Brain. Nutrients 10:N/A (2018). PubMed: 30423806
- Verma D et al. Pancreatic polypeptide and its central Y4 receptors are essential for cued fear extinction and permanent suppression of fear. Br J Pharmacol 173:1925-38 (2016). IHC . PubMed: 26844810
- Kerman IA et al. Distinct populations of presympathetic-premotor neurons express orexin or melanin-concentrating hormone in the rat lateral hypothalamus. J Comp Neurol 505:586-601 (2007). PubMed: 17924541
- Yao ST et al. Water deprivation increases the expression of neuronal nitric oxide synthase (nNOS) but not orexin-A in the lateral hypothalamic area of the rat. J Comp Neurol 490:180-93 (2005). IHC-FoFr . PubMed: 16052497
