製品の概要

  • 製品名
    Anti-OPA1 antibody [1E81D9]
    OPA1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1E81D9] to OPA1
  • 由来種
    Mouse
  • アプリケーション
    適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human OPA1 aa 1-957. The exact sequence is proprietary. The immunogen was a bacterially produced. The epitope site is unknown.
    Sequence:

    MWRLRRAAVACEVCQSLVKHSSGIKGSLPLQKLHLVSRSIYHSHHPTLKL QRPQLRTSFQ QFSSLTNLPLRKLKFSPIKYGYQPRRNFWPARLATRLL KLRYLILGSAVGGGYTAKKTFD QWKDMIPDLSEYKWIVPDIVWEIDEY IDFEKIRKALPNSEDLVKLAPDFDKIVESLSLLK DFFTSGSPEETAFR ATDRGSESDKHFRKVSDKEKIDQLQEELLHTQLKYQRILERLEKEN KE LRKLVLQKDDKGIHHRKLKKSLIDMYSEVLDVLSDYDASYNTQDHLPRVV VVGDQSAG KTSVLEMIAQARIFPRGSGEMMTRSPVKVTLSEGPHHVAL FKDSSREFDLTKEEDLAALR HEIELRMRKNVKEGCTVSPETISLNVKG PGLQRMVLVDLPGVINTVTSGMAPDTKETIFS ISKAYMQNPNAIILCI QDGSVDAERSIVTDLVSQMDPHGRRTIFVLTKVDLAEKNVASPS RIQQ IIEGKLFPMKALGYFAVVTGKGNSSESIEAIREYEEEFFQNSKLLKTSML KAHQVT TRNLSLAVSDCFWKMVRESVEQQADSFKATRFNLETEWKNNY PRLRELDRNELFEKAKNE ILDEVISLSQVTPKHWEEILQQSLWERVST HVIENIYLPAAQTMNSGTFNTTVDIKLKQW TDKQLPNKAVEVAWETLQ EEFSRFMTEPKGKEHDDIFDKLKEAVKEESIKRHKWNDFAED SLRVIQ HNALEDRSISDKQQWDAAIYFMEEALQARLKDTENAIENMVGPDWKKRWL YWKN RTQEQCVHNETKNELEKMLKCNEEHPAYLASDEITTVRKNLESR GVEVDPSLIKDTWHQV YRRHFLKTALNHCNLCRRGFYYYQRHFVDSEL ECNDVVLFWRIQRMLAITANTLRQQLTN TEVRRLEKNVKEVLEDFAED GEKKIKLLTGKRVQLAEDLKKVREIQEKLDAFIEALH

  • 特記事項

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab119685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 112 kDa.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ターゲット情報

  • 機能
    Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
  • 組織特異性
    Highly expressed in retina. Also expressed in brain, testis, heart and skeletal muscle. Isoform 1 expressed in retina, skeletal muscle, heart, lung, ovary, colon, thyroid gland, leukocytes and fetal brain. Isoform 2 expressed in colon, liver, kidney, thyroid gland and leukocytes. Low levels of all isoforms expressed in a variety of tissues.
  • 関連疾患
    Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) [MIM:165500]. OPA1 is a dominantly inherited optic neuropathy occurring in 1 in 50,000 individuals that features progressive loss in visual acuity leading, in many cases, to legal blindness.
    Defects in OPA1 are the cause of optic atrophy 1 with deafness (OPA1D) [MIM:125250]. Some individuals with mutations in OPA1 manifest also ophthalmoplegia and myopathy.
  • 配列類似性
    Belongs to the dynamin family.
  • 翻訳後修飾
    PARL-dependent proteolytic processing releases an antiapoptotic soluble form not required for mitochondrial fusion.
  • 細胞内局在
    Mitochondrion inner membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Dynamin like 120 kDa protein antibody
    • Dynamin like 120 kDa protein, mitochondrial antibody
    • Dynamin-like 120 kDa protein, form S1 antibody
    • FLJ12460 antibody
    • Juvenile kjer type optic atrophy antibody
    • KIAA0567 antibody
    • KJER type antibody
    • Large GTP binding protein antibody
    • largeG antibody
    • MGM1 antibody
    • Mitochondrial dynamin like 120 kDa protein antibody
    • Mitochondrial dynamin like GTPase antibody
    • NPG antibody
    • NTG antibody
    • OAK antibody
    • OPA 1 antibody
    • opa1 antibody
    • OPA1 gene antibody
    • OPA1_HUMAN antibody
    • Optic atrophy 1 (autosomal dominant) antibody
    • OPTIC ATROPHY 1 antibody
    • Optic atrophy 1 gene protein antibody
    • Optic atrophy 1 homolog (human) antibody
    • Optic atrophy protein 1 antibody
    • Optic atrophy protein 1 homolog antibody
    see all

画像

  • Overlay histogram showing SH-SY5Y cells stained with ab119685 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119685, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • All lanes : Anti-OPA1 antibody [1E81D9] (ab119685) at 1 µg/ml

    Lane 1 : whole cell lysates from HeLa cells(human)
    Lane 2 : whole cell lysates from H4IIE cells(rat)
    Lane 3 : whole cell lysates from MEF cells(mouse)

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP goat anti-mouse at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 112 kDa

参考文献

This product has been referenced in:
  • Takagi S  et al. Ipragliflozin improves mitochondrial abnormalities in renal tubules induced by a high-fat diet. J Diabetes Investig N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 29352520) »
  • Vishnyakova PA  et al. Alterations in antioxidant system, mitochondrial biogenesis and autophagy in preeclamptic myometrium. BBA Clin 8:35-42 (2017). Read more (PubMed: 28736722) »

See all 3 Publications for this product

レビューと Q&A

The epitope for this antibody hasn’t been mapped but the immunogen was the C-terminal region common to all of the OPA1 splice variants. Therefore, the antibody is expected to bind to all splice variants.

There are 8 different splice iso...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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