Anti-Oligodendrocyte Specific Protein 抗体 - Oligodendrocyte Marker (ab7474)
Key features and details
- Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
Oligodendrocyte Specific Protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker -
由来種
Rabbit -
特異性
Reacts specifically with 22 kDa protein derived from CNS samples. -
アプリケーション
適用あり: IHC-P, IHC-Frmore details -
種交差性
交差種: Mouse, Rat
交差が予測される動物種: Human -
免疫原
A 15 residue synthetic C-terminal peptide.
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特記事項
The concentration depends on the batch, please enquire for current stock concentration.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab7474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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IHC-P | 1/50 - 1/500. | |
IHC-Fr | Use at an assay dependent concentration. |
ターゲット情報
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機能
Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity. -
配列類似性
Belongs to the claudin family. -
細胞内局在
Cell junction > tight junction. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 5010 Human
- Entrez Gene: 18417 Mouse
- Entrez Gene: 84588 Rat
- Omim: 601326 Human
- SwissProt: O75508 Human
- SwissProt: Q60771 Mouse
- SwissProt: Q99P82 Rat
- Unigene: 31595 Human
see all -
別名
- Claudin 11 antibody
- Claudin-11 antibody
- CLD11_HUMAN antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)This image is courtesy of an Abreview submitted by Dr Qin WenImmunohistochemical analysis of adult mouse testis tissue, staining Oligodendrocyte Specific Protein (red) with ab7474.
Tissue was blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA) for 1 hour at 25°C. A TRITC-conjugated donkey anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody. -
Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
データシートおよび資料
参考文献 (12)
ab7474 は 12 報の論文で使用されています。
- Wellman SM et al. Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces. Front Neurosci 13:493 (2019). PubMed: 31191216
- Raynald et al. Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats. CNS Neurosci Ther 25:951-964 (2019). PubMed: 31486601
- Pan J et al. Expression of claudin-11 in a rat model of varicocele and its effects on the blood-testis barrier. Mol Med Rep 18:5647-5651 (2018). PubMed: 30365105
- Zhao X et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. Nat Commun 8:602 (2017). PubMed: 28928459
- Matsunaga E et al. Identification of tool use acquisition-associated genes in the primate neocortex. Dev Growth Differ 57:484-95 (2015). PubMed: 26173833