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  1. Link

    oligodendrocyte-specific-protein-antibody-oligodendrocyte-marker-ab7474.pdf

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Neuroscience Cell Type Marker Glia marker Oligodendrocyte marker
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Anti-Oligodendrocyte Specific Protein 抗体 - Oligodendrocyte Marker (ab7474)

  • Datasheet
Reviews (3)Q&A (18)References (13)

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保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
  • Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)

Key features and details

  • Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Oligodendrocyte Specific Protein (ab131748)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
一次抗体
Product image
Anti-GFAP antibody [EPR1034Y] (ab68428)

関連製品

製品の概要

  • 製品名

    Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker
    Oligodendrocyte Specific Protein 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Oligodendrocyte Specific Protein - Oligodendrocyte Marker
  • 由来種

    Rabbit
  • 特異性

    Reacts specifically with 22 kDa protein derived from CNS samples.
  • アプリケーション

    適用あり: IHC-P, IHC-Frmore details
  • 種交差性

    交差種: Rat, Human
  • 免疫原

    A 15 residue synthetic C-terminal peptide.

  • 特記事項

    The concentration depends on the batch, please enquire for current stock concentration.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Constituent: Whole serum
  • Concentration information loading...
  • 精製度

    Whole antiserum
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Oligodendrocyte marker
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Other

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Oligodendrocyte Specific Protein (ab131748)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab7474の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (2)
1/50 - 1/500.
IHC-Fr (1)
Use at an assay dependent concentration.
特記事項
IHC-P
1/50 - 1/500.
IHC-Fr
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.
  • 配列類似性

    Belongs to the claudin family.
  • 細胞内局在

    Cell junction > tight junction. Cell membrane.
  • Target information above from: UniProt accession O75508 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 5010 Human
    • Entrez Gene: 84588 Rat
    • Omim: 601326 Human
    • SwissProt: O75508 Human
    • SwissProt: Q99P82 Rat
    • Unigene: 31595 Human
    • Unigene: 8282 Rat
    • 別名

      • Claudin 11 antibody
      • Claudin-11 antibody
      • CLD11_HUMAN antibody
      • Cldn11 antibody
      • Oligodendrocyte transmembrane protein antibody
      • Oligodendrocyte-specific protein antibody
      • OSP antibody
      • OTM antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
      Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)This image is courtesy of an Abreview submitted by Dr Qin Wen
      Immunohistochemical analysis of adult mouse testis tissue, staining Oligodendrocyte Specific Protein (red) with ab7474.

      Tissue was blocked with 5% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 1% BSA) for 1 hour at 25°C. A TRITC-conjugated donkey anti-rabbit polyclonal IgG (1/200) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
      Immunohistochemistry (Frozen sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
      Immunofluorescent staining for OSP obtained with OSP antibody (ab7474) in rat brain cortex. The picture shows oligodendrocyte immunostaining in the cingular cortex. Picture taken with X20 objective, cells stained are ~30microns in diameter. Animals were intracardially perfused with 4% PFA. Tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. Protocol : free floating IHC on 30um cryostat sections. Primary antibody ab7474 was used at 1/100 and incubated overnight at RT. Secondary antibody, Alexa fluor 488 at 1/1000, incubated for 2h at RT.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker (ab7474)
      IHC image of ab7474 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab7474, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    プロトコール

    • Immunohistochemistry protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (13)

    ab7474 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab7474 は 13 報の論文で使用されています。

    • Wellman SM  et al. Revealing Spatial and Temporal Patterns of Cell Death, Glial Proliferation, and Blood-Brain Barrier Dysfunction Around Implanted Intracortical Neural Interfaces. Front Neurosci 13:493 (2019). PubMed: 31191216
    • Raynald  et al. Polypyrrole/polylactic acid nanofibrous scaffold cotransplanted with bone marrow stromal cells promotes the functional recovery of spinal cord injury in rats. CNS Neurosci Ther 25:951-964 (2019). PubMed: 31486601
    • Pan J  et al. Expression of claudin-11 in a rat model of varicocele and its effects on the blood-testis barrier. Mol Med Rep 18:5647-5651 (2018). PubMed: 30365105
    • Zhao X  et al. Neutrophil polarization by IL-27 as a therapeutic target for intracerebral hemorrhage. Nat Commun 8:602 (2017). PubMed: 28928459
    • Tikka S  et al. Proteomic Profiling in the Brain of CLN1 Disease Model Reveals Affected Functional Modules. Neuromolecular Med 18:109-33 (2016). PubMed: 26707855
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M citric acide/sodium citrate
    Sample
    Sheep Tissue sections (1-2 month old testis)
    Specification
    1-2 month old testis
    Permeabilization
    No
    Fixative
    4%PFA
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jan 19 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
    Sample
    Human Tissue sections (spinalcord)
    Specification
    spinalcord
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Feb 27 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Oligodendrocyte Specific Protein antibody - Oligodendrocyte Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (adult testes)
    Specification
    adult testes
    Permeabilization
    No
    Fixative
    4 %
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Qing Wen

    Verified customer

    投稿 Dec 24 2012

    Question



    Inquiry: AB 7474 I want to know which cell is stained by AB7474. Oligodendrocyte only? Or neuron? Glial cell like astrocyte?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 08 2013

    Answer

    Thank you for contacting us.
    Oligodendrocyte-specific protein (OSP) is a putative four-transmembrane protein of 22kD. It is primarily expressed in oligodendrocytes of the CNS and Sertoli cells of testes in the adult mouse. OSP is the third most abundant CNS myelin protein and contributes to 7% of the total myelin protein. OSP shares sequence homology with the claudin family of tight junction (TJ) proteins. It forms TJs in cell culture and co-localizes with structures similar to TJs in myelin. Thus, it was renamed OSP/Claudin-11. OSP/Claudin-11-null mice lack intramembranous junctions in between Sertoli cells in testes and within CNS myelin sheaths, producing reproductive and neurological deficiencies. This finding indicates that OSP/Claudin-11 is necessary for the formation of TJs. OSP/Claudin-11 expression is highly regulated during development, suggesting that it may play an important role in growth and differentiation of oligodendrocytes and other cells outside the CNS. OSP/Claudin-11 forms a complex with a novel member of the tetraspanin superfamily (OAP1) and b1 integrin. This egulates proliferation and migration of oligodendrocytes, a process essential for normal myelination and repair. Claudin-11 is also expressed in the epithelial tight junctions of the choroids plexus. OSP/Claudin-11 has been implicated as an autoantigen in the development of autoimmune demyelinating disease.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Feb 08 2013

    Question

    The Invitrogen secondary antibodies were:

    A21449, Alexa Fluor 647, goat anti-chicken:used with your MAP-2 targeting ab (ab5392)

    A21448, Alexa Fluor 647, donkey anti-sheep: used with your GFAP targeting ab (ab90601) (never used together with the previous ab in a same staining, obviously)

    A11008, Alexa Fluor 488, goat anti-rabbit:used with your OSP targeting ab (ab7474)

    A10036, Alex Fluor 546, donkey anti-mouse:used to target or protein of interest in CNS cells

    Hope this time you have everything you need.

    Please find attached an example of staining obtained with your primary antibodies. It is a .tif format. The staining has been performed on a brain section of human biopsy.

    I just send you one example because all the staining looks pretty similar with your antibodies.

    In red: a working antibody used to target our protein of interest, revealed with an Invitrogen antibody (A10036) (good staining)

    In green: the OSP staining (bad staining, often co-staining in nuclei only, smearing)

    In yellow: the GFAP staining (absent, even though astrocytes are for sure present in the section stained)

    The yellow staining is absent. If we modify the settings to increase the signal, the yellow staining becomes to appear also in the AbII (secondary antibody only) staining. This true for all staining to have optimal settings and avoid false positive signals. In general, MAP-2 was a little bit better than GFAP but not satisfactory for us either (not shown in the pictures forwarded to you). The data are brut from the confocal, I didn't modify them afterwards to emphasize the staining quality.

    Sincerly,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 06 2012

    Answer

    Thank you for your message and for providing this further information and the images.

    Reviewing the details, I appreciate the time you have spent on these experiments and would be pleased to arrange afree of charge replacementor credit note in compensation.

    I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Sep 06 2012

    Question

    Ok I understand your inquiries, but please, I hope there will be no more new people I will have to discuss with.

    I will answer step by step to each of your concerns:

    1. Yes indeed, it has been ordered through Lucerna Chem company. I just called them and the ordering number they gave me was the B120505 (is it possible, as you gave me the no B120502 which is really close...?). The date of order was indeed the 8th of June.

    2. Indeed, we could rule out a secondary antibody problem since they work properly with other antibodies and, furthermore when they are used alone without primary antibody (neg control), no staining can be detected with the proper settings for our samples.

    We have been using Invitrogen secondary antibodies, Alexa Fluor (647, 488 and 546) chromophore-linked, which is the most effective and suitable for immunofluorescence.

    3. Each time that we switched to a new tissue/cell type, we tested all antibodies on it. We use only human cells. I tested the antibodies on CNS tumor cell lines, then on human brain tissue sections and finally on human brain primo-cultures.

    To note: of course, when we coupled several primary and then secondary antibodies in a staining, we carefully avoided any cross-reactivity among the antibodies (primary-primary, secondary-secondary and primary-secondary) regarding the hosts.

    4. I can definitely provide pictures, but for this I will have to send another e-mail to you tomorrow, since it will take a bit of time to select the pictures and organize them in a power-point folder, format readable for you.

    In the meantime, feel free to ask further questions if my reply did not give all answers to your inquiries.

    All the best,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 05 2012

    Answer

    Thank you for your reply.

    I look forward to receiving the images, I appreciate your cooperation.

    I would appreciate if you are also able to provide further information regarding the secondary antibodies, as previously requested. The most helpful would be if you are able to provide theproduct codes or catalog numbers, and details of which primary they were used with.

    Many thanks for your time. I look forward to hearing fromyou.

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2012

    Question

    As already explained, the 3 antibodies gave the same smearing unspecific stainings in all conditions tested, it is the ab90601, the ab7474 and the ab5392.

    Thank you for your prompt reply. I appreciate that Abcam admits the problem and kindly refounds the purchase.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 04 2012

    Answer

    Thank you for your reply and for kindly providing this further information.

    Reviewing the details, I fully understand your concerns and itregrettable the results fromthese three antibodies have not been satisfactory. It is unusual for 3 antibodies to be giving the same non specific results.Therefore, before proceeding in this case, I would appreciate if you are able to provide some further information which will help my final investigations of this case so we can bring this to a close. This will also be vital for our quality monitoring.

    1. Please confirm if you have ordered through Lucerna Chem? In this case, they will be able to provide you with thePO number they used when placing the order with us. I would appreciate if you could contact them and ask them to confirm the order number and date of purchase. Was this B120502 ordered 8th June?

    2. Could you confirm if the current vial of secondary antibodies are working well with other primary antibodies? What are the results of the no primary controls for the secondaries? Which secondary antibodies have you been using?

    3. Have you been using the same type of samples (same species and tissue) for all three antibodies? If not, please provide further details.

    4. I would appreciate if you are able to provide images which will help me to assess the results.

    Thank you for your continued cooperation. I look forward to hearing from you with the requested details and hope we can resolve this case as soon as possible.

    Read More

    Abcam Scientific Support

    Answered on Sep 04 2012

    Question

    Ok, fine. But I need to fix this problem as soon as possible to be able to go further in my project.

    Please find a solution to compensate the trouble at your earliest convenience.

    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2012

    Answer

    Thank you for your patience. My colleague is still out of office this week.

    As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    In order to proceed with your complaint I would be very grateful to know the following information.

    - Could you please confirm your order details (date of purchase, Abcam Order Number or your PON)

    - Please also confirm if you are having problem with only ab7474 or other products?

    I look forward to hearing from you soon.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2012

    Question

    I will answer to each for your comments:

    - I forgot to mention it, but we added 5% goat serum to the PBS 5% FCS 0.2% saponin

    - Obviously, we titrated both the first and second antibodies before running experiments, we therefore think that the antibody dilutions are optimal.

    - Is it bad for the cells or staining to let the PFA 4% for too long? My supervisor did a lot of confocal in her previous lab and used the protocol I forwarded to you.

    - Washing the cells with PBS-Tween would not be recommended for our experiments since we co-stain cell surface receptor in addition of the ICS performed to discriminate CNS cell types.

    Regarding the results, don't you think the problems come from the antibodies themselves? How can we rule out that the problem is not antibody dependant?

    Again, would you suggest better targets than GFAP, OSP and MAP-2 to respectively stain astrocytes, oligodendrocytes and neurons? May you send aliquots to us in order to test them?

    Sincerely,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thank you for your email.

    Fixing in paraformaldehyde for more than 10-15 min will cross link the proteins to the point where antigen retrieval may be required to ensure the antibody has free access to bind and detect the protein. There is also possibility of non-specific binding of antibody and false results.

    It is indeed possible that problem might be due to antibodies however we can never be sure without doing experimental optimizations; also when multiple antibodies fail, the possibility of problem with the protocol or sample is more than antibodies. I would strongly suggest doing a quick experiment with just 10-15 minutes of PFA fixation. If the results do not improve then replacement antibody can be tried.

    GFAP is a best astrocyte marker however GLT-1, GLAST, S100 or SC1 can also be tried.

    Oligodendrocyte markers could be ab Olig2, CNPAse, Myelin-Oligodendrocyte Glycoprotein (MOG), Carbonic Anhydrase II or Microtubule-Associated Protein 4 etc.

    Doublecortin, Tyrosine Hydroxylase, BLBP, Calbindin, PGP9.5, GAD67 or GAP43 are other neuronal markers.

    PBS plus saponin is fine for antibodies dilution or permeabilization.

    I hope these suggestions would be helpful. Should the results do not improve please do not hesitate to contact me for further assistance.

    Read More

    Abcam Scientific Support

    Answered on Jul 16 2012

    Question

    Product code: 7474
    Lot number: GR9169-5
    Inquiry: cf: attached document

    Read More

    Abcam community

    Verified customer

    Asked on Jul 13 2012

    Answer

    Thank you for your email and I am sorry to hear about the problem you are experiencing.

    The protocol seems to fine to me, which is pretty much straight forward so the problem might be with the negative control cells you have chosen; these might not be a proper negative control for Claudin 11. There are publications that actually explains or mention the claudin 11 expression in PBMCs. Please check the links below;

    http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2169001/

    http://jvi.asm.org/content/early/2007/09/05/JVI.01457-07.full.pdf

    http://jvi.asm.org/content/early/2007/09/05/JVI.01457-07.full.pdf

    The gene expression profile of PBMCs (NK cells) shows the CLDN11 gene is expressed by PBMC. www.nature.com/ni/journal/v12/n8/extref/ni.2067-S5.xlsx

    The GFAP antibody ab90601 is well characterized and is guaranteed for ICC/IF. I would suggest doingfollowing protocol optimizations for better results
    - 10% Normal serum or 3%BSA with 1-2 hours incubation for blocking
    - Higher dilution of primary or secondary antibody
    - Fixations of cells with 4% PFA for 10-15 minutes
    - Washing cells in PBST for5 minutes wash X 3 times.

    I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Jul 13 2012

    1-10 of 21 Abreviews or Q&A

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