Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
50 µg
Specification
Cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 1x, Tween-20 0.1%
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
LiCor Odyssey imaging
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 32 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 06 2021