Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 [EPR12713] (ab177941)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713]
OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12713] to OGT / O-Linked N-Acetylglucosamine Transferase -
由来種
Rabbit -
特異性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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アプリケーション
適用あり: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
適用なし: IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A549, HeLa, and 293T cell lysates, Mouse heart and pancreas, Rat heart. IHC-P: human breast carcinoma tissue and lung carcinoma tissue. Flow Cyt (intra): K562 cells. ICC/IF: A549 cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12713 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Alexa Fluor® 647 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab198532)
- Alexa Fluor® 555 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab215809)
- Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] - BSA and Azide free (ab236014)
- Alexa Fluor® 568 Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab312586)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab177941の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB | (2) |
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa).
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ICC/IF |
1/50 - 1/100.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
特記事項 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 116 kDa). |
ICC/IF
1/50 - 1/100. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
ターゲット情報
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機能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
組織特異性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
ドメイン
The TPR repeat domain mediates recognition of protein substrates. -
翻訳後修飾
Ubiquitinated, leading to its proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 26295 Rat
- Omim: 300255 Human
- SwissProt: O15294 Human
- SwissProt: Q8CGY8 Mouse
- SwissProt: P56558 Rat
- Unigene: 405410 Human
see all -
別名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
画像
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Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) cells labeling OGT / O-Linked N-Acetylglucosamine Transferase with purified ab177941 at 1:40 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling OGT / O-Linked N-Acetylglucosamine Transferase using ab177941. The cells were fixed with 4% paraformaldehyde then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab177941 at 1:100 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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Immunohistochemical analysis of Paraffin-embedded sections human lung carcinoma tissue labelling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1:2000 dilution (0.2 μg/ml), followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human lung carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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Immunohistochemical analysis of Paraffin-embedded sections human breast carcinoma tissue labelling OGT / O-Linked N-Acetylglucosamine Transferase with ab177941 at 1:2000 dilution (0.2 μg/ml), followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human breast carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody [EPR12713] (ab177941) at 1/10000 dilution
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
Lane 4 : Mouse heart
Lane 5 : Mouse pancreas
Lane 6 : Rat heart
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
ab181602 was used as GAPDH loading control.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab177941 は 21 報の論文で使用されています。
- Zhu G et al. O-GlcNAcylation enhances sensitivity to RSL3-induced ferroptosis via the YAP/TFRC pathway in liver cancer. Cell Death Discov 7:83 (2021). PubMed: 33863873
- Oliveira-Nunes MC et al. O-GlcNAcylation protein disruption by Thiamet G promotes changes on the GBM U87-MG cells secretome molecular signature. Clin Proteomics 18:14 (2021). PubMed: 33902430
- Li C et al. Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866. Sci Rep 11:11272 (2021). PubMed: 34050207
- Wu J et al. Melatonin reduces proliferation and promotes apoptosis of bladder cancer cells by suppressing O-GlcNAcylation of cyclin-dependent-like kinase 5. J Pineal Res 71:e12765 (2021). PubMed: 34487576
- Kalantzakos TJ et al. MiRNA-424-5p Suppresses Proliferation, Migration, and Invasion of Clear Cell Renal Cell Carcinoma and Attenuates Expression of O-GlcNAc-Transferase. Cancers (Basel) 13:N/A (2021). PubMed: 34680309