Anti-OGT / O-Linked N-Acetylglucosamine Transferase 抗体 (ab96718)
Key features and details
- Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase
- Suitable for: ICC/IF, WB, IHC-P, IP
- Reacts with: Mouse, Rat, Human, Zebrafish
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody
OGT / O-Linked N-Acetylglucosamine Transferase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to OGT / O-Linked N-Acetylglucosamine Transferase -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IHC-P, IPmore details -
種交差性
交差種: Mouse, Rat, Human, Zebrafish
交差が予測される動物種: Cow, Pig -
免疫原
Recombinant fragment corresponding to Human OGT/ O-Linked N-Acetylglucosamine Transferase aa 213-462.
Database link: O15294 -
ポジティブ・コントロール
- WB: HeLa, PC-12 cells. Mouse brain tissue extracts. ICC/IF: MCF7, DU145, HeLa cells. IHC-P: Cal27 Xenograft. Rat hind brain. IP: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96718の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB | (8) |
1/500 - 1/3000. Predicted molecular weight: 117 kDa.
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IHC-P | (1) |
1/100 - 1/1000.
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IP |
1/100 - 1/500.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/3000. Predicted molecular weight: 117 kDa. |
IHC-P
1/100 - 1/1000. |
IP
1/100 - 1/500. |
ターゲット情報
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機能
Addition of nucleotide-activated sugars directly onto the polypeptide through O-glycosidic linkage with the hydroxyl of serine or threonine. Mediates the O-glycosylation of MLL5 and HCFC1. Promotes proteolytic maturation of HCFC1. -
組織特異性
Highly expressed in pancreas and to a lesser extent in skeletal muscle, heart, brain and placenta. Present in trace amounts in lung and liver. -
パスウェイ
Protein modification; protein glycosylation. -
配列類似性
Belongs to the O-GlcNAc transferase family.
Contains 13 TPR repeats. -
ドメイン
The TPR repeat domain mediates recognition of protein substrates. -
翻訳後修飾
Ubiquitinated, leading to its proteasomal degradation. -
細胞内局在
Cytoplasm. Nucleus. Mostly in the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 532053 Cow
- Entrez Gene: 8473 Human
- Entrez Gene: 108155 Mouse
- Entrez Gene: 664652 Pig
- Entrez Gene: 26295 Rat
- Entrez Gene: 337685 Zebrafish
- Omim: 300255 Human
- SwissProt: O15294 Human
see all -
別名
- FLJ23071 antibody
- GlcNAc transferase antibody
- HRNT1 antibody
see all
画像
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Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 117 kDa
7.5% SDS PAGE -
All lanes : Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Zebrafish heart homogenate at 20 µg
Lane 4 : Zebrafish liver homogenate at 20 µg
Lane 5 : Zebrafish skeletal muscle homogenate at 20 µg
Lane 6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 117 kDa
Observed band size: 117 kDa
Exposure time: 5 minutes -
HeLa cells stained for O-GlcNAc (green) using ab96718 at 1/200 dilution in ICC/IF.
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Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using ab96718 at 1/100 dilution.
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Immunoprecipitation analysis of OGT/O-linked N-Acetylglucosamine Transferase protein from A431 whole cell extracts using ab96718 (5µg). Western blot analysis was performed using ab96718 and an Easyblot anti-rabbit IgG was used as the secondary antibody.
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Ab96718 staining OGT / O-Linked N-Acetylglucosamine Transferase in MCF7 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). MFC7 cells were fixed with paraformaldehyde at room temperature for 15 minutes. Samples were incubated with primary antibody at a 1:500 dilution.
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Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/1000 dilution + PC-12 whole cell extract at 30 µg
Predicted band size: 117 kDa5% SDS-PAGE
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ICC/IF image of ab96718 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96718, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Paraffin embedded rat hind brain tissue stained for O-GlcNAc using ab96718 at 1/500 in immunohistochemical analysis.
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Anti-OGT / O-Linked N-Acetylglucosamine Transferase antibody (ab96718) at 1/2000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 117 kDa7.5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab96718 は 36 報の論文で使用されています。
- Yu F et al. Dynamic O-GlcNAcylation coordinates ferritinophagy and mitophagy to activate ferroptosis. Cell Discov 8:40 (2022). PubMed: 35504898
- Lee HW et al. Targeted Inhibition of O-Linked β-N-Acetylglucosamine Transferase as a Promising Therapeutic Strategy to Restore Chemosensitivity and Attenuate Aggressive Tumor Traits in Chemoresistant Urothelial Carcinoma of the Bladder. Biomedicines 10:N/A (2022). PubMed: 35625898
- Wang TF et al. Disruption of O-GlcNAcylation Homeostasis Induced Ovarian Granulosa Cell Injury in Bovine. Int J Mol Sci 23:N/A (2022). PubMed: 35887161
- Xiong X et al. Intestinal Epithelial STAT6 Activation Rescues the Defective Anti-Helminth Responses Caused by Ogt Deletion. Int J Mol Sci 23:N/A (2022). PubMed: 36232438
- Cao Y et al. O-GlcNAcylation of SIRT1 Protects against Cold Stress-Induced Skeletal Muscle Damage via Amelioration of Mitochondrial Homeostasis. Int J Mol Sci 23:N/A (2022). PubMed: 36498847