Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP5421] to Oct6 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
製品名Anti-Oct6 antibody [EP5421] - BSA and Azide free
Oct6 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EP5421] to Oct6 - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,ICC/IF,IHC-P or IP
種交差性交差種: Mouse, Human
Synthetic peptide within Human Oct6. The exact sequence is proprietary.
ab248163 is the carrier-free version of ab126746 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248163 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 2.87 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248163 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
機能Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Thought to be involved in early embryogenesis and neurogenesis.
組織特異性Expressed in embryonal stem cells and in the developing brain.
配列類似性Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain.
- Information by UniProt
- class 3 antibody
- Oct-6 antibody
- OCT6 antibody
All lanes : Anti-Oct6 antibody [EP5421] (ab126746) at 1/1000 dilution
Lane 1 : F9 cell lysate
Lane 2 : NCCIT cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 45 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
This data was developed using ab126746, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.