Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (in vitro produced preimplantation embryo day 7)
Permeabilization
Yes - 0.5 % Triton X 100
Specification
in vitro produced preimplantation embryo day 7
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS with 0.1% polyvinylalcohol
Dilution
1/250
Additional data
Additional Notes
Reported in the literature is expression of OCT4 in both the trophectoderm and the inner cell mass of the bovine blastocyst. This antibody apparently only reacts against OCT4 in the inner cell mass.
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投稿 Dec 07 2018