Application
Western blot
Sample
Human Cell lysate - whole cell (Human embryonic stem cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE gel)
Loading amount
15 µg
Specification
Human embryonic stem cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 2% milk in TBS with 0.1% Tween20
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Anti-tubulin was used as a loading control (non-abcam antibody).
Exposure
30 second(s)
Bands
Specific: 45 kDa
Positive control
Two human embryonic stem cell lines (H7.s14 and H7.s6).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Joanne Lacey
Verified customer
投稿 Aug 07 2017