Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced pluripotent cells)
Permeabilization
Yes - 0.5% TX100
Specification
induced pluripotent cells
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TX100, 1% goat serum, 1X PBS
Dilution
1/500
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Oct4 is located in nuclei and is labaled green. Nuclei were stained with DAPI.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
投稿 Nov 04 2016