Application
Western blot
Sample
Human Tissue lysate - whole (293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
293T
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: pierce Goat anti-mouse IgG,(H+L),peroxidase conjug
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Overexpression of OGT
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
Positive control
not overexpression of OGT
Additional data
Additional Notes
When we overexpressed the glycosylated enzyme OGT, the bands became darker, so we assumed that the glycosylated antibodies were effective. it's hard to define the exact molecular weight band
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Yuanxin Yang
Verified customer
投稿 Feb 02 2021