Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Human Cell lysate - whole cell (T lymphocyte (human))
Specification
T lymphocyte (human)
Treatment
10,20,50 mM D-Galactopyranosyl-b-D-thiogalactopyranoside for 1hr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/5000
Secondary antibody
Name
Non-Abcam antibody was used: Horse Anti-mouse IgG, HRP-linked Antibody
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: many bands kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
Media alone
Positive control
Jurkat T lymphocytes
Additional data
Additional Notes
We used PVDF membrane and performed under reducing conditions.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 12 2015