Anti-O-Linked N-Acetylglucosamine 抗体 [RL2] (ab2739)
Key features and details
- Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-O-Linked N-Acetylglucosamine antibody [RL2]
O-Linked N-Acetylglucosamine 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RL2] to O-Linked N-Acetylglucosamine -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Tissue, cells or virus corresponding to O-Linked N-Acetylglucosamine. Specifically, isolated rat liver nuclear envelopes, which contain 8 O-Linked glycoproteins in the nuclear pore complex
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ポジティブ･コントロール
- ICC-IF: MCF7 cells. WB: Jurkat cells treated with 50 uM PugNAc; SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac; Rat Liver Nuclear Envelope lysate.
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特記事項
This antibody clone [RL2] is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
精製度
IgG fraction -
ポリ/モノ
モノクローナル -
クローン名
RL2 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Alternative Versions
- Anti-O-Linked N-Acetylglucosamine antibody [RL2] (Alexa Fluor® 488) (ab201993)
- Anti-O-Linked N-Acetylglucosamine antibody [RL2] (Alexa Fluor® 647) (ab201994)
- Anti-O-Linked N-Acetylglucosamine antibody [RL2] (HRP) (ab201995)
- Anti-O-Linked N-Acetylglucosamine antibody [RL2] - BSA and Azide free (ab264496)
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab2739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF | Use a concentration of 5 - 10 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. |
ターゲット情報
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関連性
Many cellular proteins, including nuclear pore, oncogene, cytoskeletal, heat shock, viral and transcription regulatory proteins contain single O-linked N-acetylglucosamine (O-GlcNAc) residues attached to serine or threonine residues. It has been observed that O-GlcNAc glycosylated proteins tend to be under phosphorylated relative to unglycosylated proteins and that O-GlcNAc bearing proteins tend to be found in multimeric complexes. This has led to the suggestion that O-GlcNAc glycosylation may obscure phosphorylation sites and acts as a signaling mechanism or mediator of signaling.
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.co.jp/ps/products/2/ab2739/Images/ab2739-279434-anti-o-linked-n-acetylglucosamine-antibody-rl2-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)ab2739 stained in MCF7 cells. Cells were fixed with 4% paraformaldehyde (10min) at room temperature and incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab2739 at 5µg/ml and ab6046 (Rabbit polyclonal to beta Tubulin - Loading Control) at 1/1000 dilution overnight at +4°C. The secondary antibodies were ab150080 (pseudo-colored red) and ab150117 (colored green) used at 1 ug/ml for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.co.jp/ps/products/2/ab2739/Images/ab2739-232645-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)All lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml
Lanes 1 & 3 : Jurkat cells treated with 0 uM PugNAc
Lane 2 : Jurkat cells treated with 50 uM PugNAc (3 hours)
Lane 4 : Jurkat cells treated with 4 mM glucosamine and 50 uM PugNAc (3 hours)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 12 minutesJurkat cells were treated with either 50 uM PugNAc (ab144670) or 4 mM glucosamine + 50 uM PugNAc (ab144670) for three hours prior to harvest to stimulate O-linked glycosylation. The expected increase in glycosylation is observed in the treated lanes 2 & 4.
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.co.jp/ps/products/2/ab2739/Images/ab2739-232732-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)This image is courtesy of an anonymous AbreviewAll lanes : Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1/3000 dilution
Lane 1 : Human neuroblastoma (SH-SY5Y) whole cell lysate - treated with 50µM z-Pugnac for 24 hours
Lane 2 : Human neuroblastoma (SH-SY5Y) whole cell lysate - untreated
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated horse anti-mouse IgG polyclonal
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 30 seconds
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![Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)](https://www.abcam.co.jp/ps/products/2/ab2739/Images/ab2739-6300-anti-o-linked-n-acetylglucosamine-antibody-rl2-western-blot.jpg)
Western blot - Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739)Anti-O-Linked N-Acetylglucosamine antibody [RL2] (ab2739) at 1 µg/ml + Rat Liver Nuclear Envelope at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
The antibody was tested against the immunogen (isolated rat liver nuclear envelopes, which contain 8 O-linked glycoproteins in the nuclear pore complex).
参考文献 (100)
ab2739 は 100 報の論文で使用されています。
- Netsirisawan P et al. Quantitative proteomic analysis of the association between decreasing O-GlcNAcylation and metastasis in MCF-7 breast cancer cells. Int J Oncol 56:1387-1404 (2020). PubMed: 32236627
- Jiang M et al. Fatty acid-induced CD36 expression via O-GlcNAcylation drives gastric cancer metastasis. Theranostics 9:5359-5373 (2019). PubMed: 31410220
- Liu B et al. The lineage stability and suppressive program of regulatory T cells require protein O-GlcNAcylation. Nat Commun 10:354 (2019). PubMed: 30664665
- Sharif S et al. Study of cross talk between phosphatases and OGA on a ZO-3-derived peptide. Amino Acids 51:739-743 (2019). PubMed: 30725225
- Huang R et al. Increased Ratio of Global O-GlcNAcylation to Tau Phosphorylation at Thr212 Site Is Associated With Better Memory Function in Patients With Type 2 Diabetes. Front Physiol 10:110 (2019). PubMed: 30837891
