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Our Abpromise guarantee covers the use of ab13851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/2000. PubMed: 21726557|
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||1/500 - 1/1000. Detects a band of approximately 84 kDa.|
Jurkat (Human T cell leukemia cell line from peripheral blood) cells stained for NUR77 using ab13851 at a dilution of 1/50 in ICC/IF. Secondary used was a Dylight®488 conjugate (green). Alpha tubulin and nuclei were counterstained against Dylight®568 (Red) and DAPI (Blue), respectively.
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