Anti-Nup107 抗体 [EPR22877-21] (ab236634)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22877-21] to Nup107
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nup107 antibody [EPR22877-21]
Nup107 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22877-21] to Nup107 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IPmore details
適用なし: Flow Cyt,ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: PC-12, RAW 264.7, NIH/3T3, HepG2, HEK-293T and HeLa whole cell lysates. IP: HEK-293T whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22877-21 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236634の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 106 kDa).
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IP |
1/30.
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特記事項 |
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WB
1/1000. Detects a band of approximately 107 kDa (predicted molecular weight: 106 kDa). |
IP
1/30. |
ターゲット情報
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機能
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. -
組織特異性
Ubiquitously expressed in fetal and adult tissues. -
関連疾患
Nephrotic syndrome 11 -
配列類似性
Belongs to the nucleoporin Nup84/Nup107 family. -
細胞内局在
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755). - Information by UniProt
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参照データベース
- Entrez Gene: 57122 Human
- Entrez Gene: 103468 Mouse
- Entrez Gene: 116555 Rat
- Omim: 607617 Human
- SwissProt: P57740 Human
- SwissProt: Q3TT06 Mouse
- SwissProt: Q3V395 Mouse
- SwissProt: Q569X8 Mouse
see all -
別名
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
see all
画像
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All lanes : Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution (Purified)
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis antibody reacts with an unidentifiable protein around 37 kDa.
Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Nup107 antibody [EPR22877-21] (ab236634) at 1/1000 dilution (Purified)
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 10 µg
Lane 2 : 293T (human embryonic kidney epithelial cell), whole cell lysate at 10 µg
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 106 kDa
Observed band size: 107 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times:
Lanes 1-2: 3 minutes; Lane 3: 15 seconds.
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Nup107 was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug with ab236634 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab236634. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: HEK-293T (human embryonic kidney epithelial cell) whole cell lysate 10ug
Lane 2: ab236634 IP in HEK-293T whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab236634 in HEK-293T whole cell lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 6 seconds
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab236634 は論文での使用が確認できていません。