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  1. Link

    numb-antibody-ab4147.pdf

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Anti-NUMB 抗体 (ab4147)

  • Datasheet
  • SDS
Reviews (10)Q&A (6)References (39)

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Western blot - Anti-NUMB antibody (ab4147)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)

Key features and details

  • Goat polyclonal to NUMB
  • Suitable for: ELISA, IHC-P, IP, WB, ICC/IF
  • Reacts with: Mouse, Chicken, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-NUMB antibody [EPR21988] (ab220362)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-NUMB antibody
    NUMB 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to NUMB
  • 由来種

    Goat
  • 特異性

    This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally.
  • アプリケーション

    適用あり: ELISA, IHC-P, IP, WB, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Chicken, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human NUMB aa 638-651 (C terminal).
    Sequence:

    C-PFSSDLQKTFEIEL


    Database link: P49757
    (Peptide available as ab22990)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells;
  • 特記事項

    This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human NUMB protein (ab131949)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4147の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-P (3)
Use at an assay dependent concentration.

See Abreview.

IP (1)
Use at an assay dependent concentration.

For 1 hr on mouse brain lysate (see Abreview).

WB (3)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).

1 hour primary incubation at room temperature is recommended for this product.

ICC/IF (3)
Use a concentration of 10 µg/ml.
特記事項
ELISA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

See Abreview.

IP
Use at an assay dependent concentration.

For 1 hr on mouse brain lysate (see Abreview).

WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa).

1 hour primary incubation at room temperature is recommended for this product.

ICC/IF
Use a concentration of 10 µg/ml.

ターゲット情報

  • 機能

    Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage.
  • 配列類似性

    Contains 1 PID domain.
  • 翻訳後修飾

    Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P49757 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 512187 Cow
    • Entrez Gene: 480381 Dog
    • Entrez Gene: 8650 Human
    • Entrez Gene: 18222 Mouse
    • Omim: 603728 Human
    • SwissProt: P49757 Human
    • SwissProt: Q9QZS3 Mouse
    • Unigene: 654609 Human
    • Unigene: 714879 Human
    • Unigene: 4390 Mouse
    see all
  • 別名

    • c14_5527 antibody
    • C14orf41 antibody
    • FLJ31314 antibody
    • h Numb antibody
    • h-Numb antibody
    • Numb antibody
    • Numb homolog (Drosophila) antibody
    • Numb homolog antibody
    • Numb protein homolog antibody
    • NUMB_HUMAN antibody
    • Protein numb homolog antibody
    • Protein S171 antibody
    • S171 antibody
    see all

画像

  • Western blot - Anti-NUMB antibody (ab4147)
    Western blot - Anti-NUMB antibody (ab4147)
    Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg

    Predicted band size: 71 kDa



    Detected by chemiluminescence.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
    Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)

    Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing cell junction and cytoplasmic staining. The nuclear stain is DAPI (blue).

    Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
    Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)

    Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing plasma membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).

    Negative control: Unimmunized goat IgG (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml).

  • Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)
    Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreview

    ab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An  Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.

    See Abreview

プロトコール

  • Recommended WB protocol for ab4147

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (39)

ab4147 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4147 は 39 報の論文で使用されています。

  • Charruyer A  et al. Decreased p53 is associated with a decline in asymmetric stem cell self-renewal in aged human epidermis. Aging Cell 20:e13310 (2021). PubMed: 33524216
  • Lau EO  et al. DIAPH3 deficiency links microtubules to mitotic errors, defective neurogenesis, and brain dysfunction. Elife 10:N/A (2021). PubMed: 33899739
  • Gomez K  et al. Non-SUMOylated CRMP2 decreases NaV1.7 currents via the endocytic proteins Numb, Nedd4-2 and Eps15. Mol Brain 14:20 (2021). PubMed: 33478555
  • Höpner SS  et al. LIGHT/LTßR signaling regulates self-renewal and differentiation of hematopoietic and leukemia stem cells. Nat Commun 12:1065 (2021). PubMed: 33594067
  • Li Y  et al. CUL4B contributes to cancer stemness by repressing tumor suppressor miR34a in colorectal cancer. Oncogenesis 9:20 (2020). PubMed: 32054830
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 16 Abreviews or Q&A

Western blot abreview for Anti-NUMB antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7 cells lysate)
Gel Running Conditions
Reduced Denaturing (4–20% precast polyacrylamide gel)
Loading amount
20 µg
Specification
MCF7 cells lysate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Apr 28 2022

Western blot abreview for Anti-NUMB antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Zebrafish Tissue lysate - whole (5 days whole tissue)
Gel Running Conditions
Reduced Denaturing (4–20% precast polyacrylamide gel)
Loading amount
30 µg
Specification
5 days whole tissue
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 08 2021

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (NIH3T3)
Specification
NIH3T3
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Mar 19 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

Inconclusive
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin equivalent)
Specification
Skin equivalent
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako EnVision Kit 5007
Permeabilization
No
Blocking step
(agent) for 20 minute(s) · Concentration: 100% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 13 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (skin equivalent, human keratinocytes Wholemount)
Specification
skin equivalent, human keratinocytes Wholemount
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% Triton-X100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 06 2012

Immunocytochemistry/ Immunofluorescence abreview for Anti-NUMB antibody

Excellent
Abreviews
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (embryonic brain tissue)
Specification
embryonic brain tissue
Fixative
Paraformaldehyde
Permeabilization
Yes - 1% TX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 09 2009

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

Poor
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryo CNS)
Specification
embryo CNS
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodiumcitrate pH 6.0, also Tris-EDTA pH 9.0 tried
Permeabilization
Yes - 0.1 % Tween-20 for 10-30 minutes
Blocking step
!% BSA and 10%donkey serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 26 2007

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NUMB antibody

Below Average
Abreviews
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryo)
Specification
embryo
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jul 13 2007

Question

Anti-NUMB antibody for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (Human)
Sample Species: Human
Type: Tissue sections
Specification: Skin equivalent
Fixative: Paraformaldehyde
Antigen retrieval step: Heat mediated
Buffer/Enzyme Used: Dako EnVision Kit 5007
Permeabilization: No
Dilution: 1/20
Incubation time: 1 hour 30 minutes at 25°C
Diluent: 3% BSA in PBS
Secondary Antibody: Dako EnVision Kit 5007
Conjugation: HRP

Read More

Abcam community

Verified customer

Asked on Jul 16 2012

Answer

Thank you for submitting an Abreview of ab4147. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

- testing various titers of primary antibody
- a different antigen retrieval solution


If you decide to follow these suggestions, please let me know if they are helpful. Additionally, this antibody is covered by our Abpromise, so if the suggestions do not help and the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

I look forward to your reply.

Read More

Abcam Scientific Support

Answered on Jul 16 2012

Question

Dear Tech support

Could you pleasespeculate regarding ab4147 reactivity against Drosophila derived samples?

Would you like to offer a participation in the abreview promotion for Drosophila with one of the Numb antibodies available.


Thank you for your kind help.

Regards

Read More

Abcam community

Verified customer

Asked on Feb 07 2012

Answer

Thank you for contacting us.


The anti-NUMB antibody (ab4147) is raised against a peptide corresponding to residues 638-651 of human NUMB. Through sequence alignment of the Drosophila melanogaster protein (SwissProt reference http://www.uniprot.org/uniprot/P16554), this region shares very little homology with the human protein. I would therefore not suggest usign this antibody to detect the Drosophila protein.


Unfortunately we have no other anti-NUMB antibodies that I can suggest as an alternative. You may have more luck by searching Biocompare:


http://www.biocomplare.com


I am sorry that I have not been able to be of more help in this instance. If you have any further questions please do not hesitate to contact us again.

Read More

Abcam Scientific Support

Answered on Feb 07 2012

1-10 of 16 Abreviews or Q&A

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