Anti-NUMB 抗体 (ab4147)
Key features and details
- Goat polyclonal to NUMB
- Suitable for: IHC-P, WB, ELISA, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-NUMB antibody
NUMB 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to NUMB -
由来種
Goat -
特異性
This antibody is expected to recognize all reported isoforms (NP_001005743.1, 001005744.1, NP_003735, NP_001005745.1). It is also likely to cross-react with Numblike (NUMBL, GeneID: 9253) which has a very similar C-Terminus, although this has not yet been tested experimentally. -
アプリケーション
適用あり: IHC-P, WB, ELISA, ICC/IFmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chicken, Cow, Dog
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免疫原
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ポジティブ･コントロール
- WB: Mouse brain lysate; ICC: A431 and NIH3T3 cells; IHC-P: Human prostate tissue.
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特記事項
This product may cross-react with Numblike (NUMBL, GeneID: 9253) Principal Names - NUMB; S171; numb homolog (Drosophila) Official Gene Symbol - NUMB GenBank Accession Number – NP_003735 LocusLink ID - 8650 (human); 18222 (mouse) Gene Ontology terms - integral to plasma membrane
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab4147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 71 kDa). 1 hour primary incubation is recommended for this product. |
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| ELISA | 1/128000. | |
| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Plays a role in the process of neurogenesis. Required throughout embryonic neurogenesis to maintain neural progenitor cells, also called radial glial cells (RGCs), by allowing their daughter cells to choose progenitor over neuronal cell fate. Not required for the proliferation of neural progenitor cells before the onset of neurogenesis. Also involved postnatally in the subventricular zone (SVZ) neurogenesis by regulating SVZ neuroblasts survival and ependymal wall integrity. May also mediate local repair of brain ventricular wall damage. -
配列類似性
Contains 1 PID domain. -
翻訳後修飾
Isoform 1 and isoform 2 are ubiquitinated by LNX leading to their subsequent proteasomal degradation (By similarity). Ubiquitinated; mediated by SIAH1 and leading to its subsequent proteasomal degradation. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 512187 Cow
- Entrez Gene: 480381 Dog
- Entrez Gene: 8650 Human
- Entrez Gene: 18222 Mouse
- Omim: 603728 Human
- SwissProt: P49757 Human
- SwissProt: Q9QZS3 Mouse
- Unigene: 654609 Human
see all -
別名
- c14_5527 antibody
- C14orf41 antibody
- FLJ31314 antibody
see all
画像
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Western blot - Anti-NUMB antibody (ab4147)Anti-NUMB antibody (ab4147) at 1 µg/ml + Mouse brain lysates at 35 µg
Predicted band size: 71 kDaDetected by chemiluminescence.
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunocytochemistry/ Immunofluorescence analysis of A431 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. -
Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NUMB with ab4147 at 10ug/mL. Alexa Fluor488 at 2ug/mL was used as the secondary antibody. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/mL) followed by Alexa Fluor488 secondary antibody (2ug/mL).
Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton.
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Immunocytochemistry/ Immunofluorescence - Anti-NUMB antibody (ab4147)This image is courtesy of an anonymous Abreviewab4147 staining NUMB in the NIH3T3 cell line from Mouse fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 10% serum for 60 minutes at 24°C. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit polyclonal(1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NUMB antibody (ab4147)Single collagenase-isolated mouse muscle fibre stained with DAPI (blue) to reveal nuclei and anti-Numb (green) which detects satellite cells.
This picture was kindly supplied as part of the review submitted by Christopher Mann.
参考文献 (29)
ab4147 は 29 報の論文で使用されています。
- Huang G et al. TUSC7 suppression of Notch activation through sponging MiR-146 recapitulated the asymmetric cell division in lung adenocarcinoma stem cells. Life Sci 232:116630 (2019). PubMed: 31279783
- Abdi K et al. EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche Differentiation. Cell Rep 28:2012-2022.e4 (2019). PubMed: 31433979
- Cheng Y et al. m6A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep 28:1703-1716.e6 (2019). PubMed: 31412241
- Kraushaar DC et al. The gene repressor complex NuRD interacts with the histone variant H3.3 at promoters of active genes. Genome Res 28:1646-1655 (2018). PubMed: 30254051
- García-Heredia JM & Carnero A NUMB and NUMBL differences in gene regulation. Oncotarget 9:9219-9234 (2018). PubMed: 29507685
