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リコンビナントRabMAb

Anti-NuMA 抗体 [EP3976] (ab109262)

Submit a review Submit a question References (15)
Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] (ab109262)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
  • Western blot - Anti-NuMA antibody [EP3976] (ab109262)
  • Anti-NuMA antibody [EP3976] (ab109262)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3976] to NuMA
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-NuMA antibody [EP3976]
    NuMA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EP3976] to NuMA

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP

  • 種交差性

    交差種: Human
    交差が予測される動物種: Mouse, Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • K562, HeLa, MCF7, Raji, SW480, CACO2, and MOLT4 cell lysates; Human breast carcinoma, normal breast and colonic adenocarcinoma tissues; HeLa cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EP3976

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109262の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 238 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
特記事項
WB
1/1000 - 1/10000. Predicted molecular weight: 238 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for Flow Cyt or IP.

ターゲット情報

  • 機能

    May be a structural component of the nucleus.

  • 細胞内局在

    Nucleus. Chromosome. Dissociates from condensing chromosomes during early prophase, before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Centrophilin stabilizes mitotic spindle in mitotic cells antibody
      • NMP 22 antibody
      • Nuclear matrix protein 22 antibody
      • Nuclear mitotic apparatus protein 1 antibody
      • Nuclear mitotic apparatus protein antibody
      • NUMA 1 antibody
      • NUMA antibody
      • NuMA protein antibody
      • NUMA1 antibody
      • NUMA1_HUMAN antibody
      • SP H antigen antibody
      • SP-H antigen antibody
      • Structural nuclear protein antibody
      see all

    画像

    • Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] (ab109262)
      Immunocytochemistry/ Immunofluorescence - Anti-NuMA antibody [EP3976] (ab109262)
      ab109262, at 1/100 dilution, staining NuMA in HeLa cells by Immunofluorescence.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

      ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human colonic adenocarcinoma by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

      ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NuMA antibody [EP3976] (ab109262)

      ab109262, at 1/250 dilution, staining NuMA in paraffin-embedded Human breast tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-NuMA antibody [EP3976] (ab109262)
      Western blot - Anti-NuMA antibody [EP3976] (ab109262)
      All lanes : Anti-NuMA antibody [EP3976] (ab109262) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : Raji cell lysate
      Lane 5 : SW480 cell lysate
      Lane 6 : CACO2 cell lysate
      Lane 7 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 238 kDa

    • Anti-NuMA antibody [EP3976] (ab109262)
      Anti-NuMA antibody [EP3976] (ab109262)

    参考文献 (15)

    ab109262 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab109262 は 15 報の論文で使用されています。

    • Chaigne A  et al. Three-dimensional geometry controls division symmetry in stem cell colonies. J Cell Sci 134:N/A (2021). PubMed: 34323278
    • Fáberová V  et al. Super-Resolution Localisation of Nuclear PI(4)P and Identification of Its Interacting Proteome. Cells 9:N/A (2020). PubMed: 32403279
    • Bonucci M  et al. mTOR and S6K1 drive polycystic kidney by the control of Afadin-dependent oriented cell division. Nat Commun 11:3200 (2020). PubMed: 32581239
    • Chinen T  et al. NuMA assemblies organize microtubule asters to establish spindle bipolarity in acentrosomal human cells. EMBO J 39:e102378 (2020). PubMed: 31782546
    • Alfonso-Pérez T  et al. MAD1-dependent recruitment of CDK1-CCNB1 to kinetochores promotes spindle checkpoint signaling. J Cell Biol 218:1108-1117 (2019). PubMed: 30674583
    View all Publications for this product

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