Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7734] to NUDT5 - BSA and Azide free
- Suitable for: IHC-P, Flow Cyt, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
製品名Anti-NUDT5 antibody [EPR7734] - BSA and Azide free
NUDT5 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR7734] to NUDT5 - BSA and Azide free
アプリケーション適用あり: IHC-P, Flow Cyt, WBmore details
適用なし: ICC/IF or IP
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human NUDT5 aa 1-100. The exact sequence is proprietary.
- WB: HEK293T, HepG2 and U-937 cell lysates.
Ab248329 is the carrier-free version of ab129172. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 1.77 x 10 -10 M Learn more about KD
Concentration information loading...
KO cell lysates
Our Abpromise guarantee covers the use of ab248329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
機能Hydrolyzes with similar activities ADP-ribose, ADP-mannose, and ADP-glucose. Can also hydrolyze other nucleotide sugars with low activity.
組織特異性Widely expressed. Most abundant in liver.
配列類似性Belongs to the Nudix hydrolase family.
Contains 1 nudix hydrolase domain.
- Information by UniProt
- ADP sugar pyrophosphatase antibody
- ADP-sugar pyrophosphatase antibody
- hYSAH 1 antibody
All lanes : Anti-NUDT5 antibody [EPR7734] (ab129172) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NUDT5 knockout HEK293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : U-937 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab129172).
ab129172 Anti-NUDT5 antibody [EPR7734] was shown to specifically react with NUDT5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266242 (knockout cell lysate ab258087) was used. Wild-type and NUDT5 knockout samples were subjected to SDS-PAGE. ab129172 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.