Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3911(2)] to Nucleostemin - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
製品名Anti-Nucleostemin antibody [EPR3911(2)] - BSA and Azide free
Nucleostemin 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR3911(2)] to Nucleostemin - BSA and Azide free
アプリケーション適用あり: ICC/IF, Flow Cyt, WBmore details
適用なし: IHC-P or IP
Synthetic peptide within Human Nucleostemin aa 500-600 (C terminal). The exact sequence is proprietary.
Database link: Q9BVP2
ab248337 is the carrier-free version of ab129185 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248337 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 6.76 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab248337 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 62 kDa.|
機能May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis.
組織特異性Increased levels in lung tissue in cancer patients.
配列類似性Belongs to the MMR1/HSR1 GTP-binding protein family.
Contains 1 G (guanine nucleotide-binding) domain.
ドメインThe basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis.
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
細胞内局在Nucleus. Nucleus > nucleolus. Shuttles between the nucleus and nucleolus.
- Information by UniProt
- C77032 antibody
- E2 induced gene 3 protein antibody
- E2-induced gene 3 protein antibody
All lanes : Anti-Nucleostemin antibody [EPR3911(2)] (ab129185) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : SW480 cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 62 kDa
This data was developed using ab129185, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.