Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt, WB
- Knockout validated
- Reacts with: Human
製品名Anti-Nucleoside phosphorylase antibody [EPR5715] - BSA and Azide free
Nucleoside phosphorylase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5715] to Nucleoside phosphorylase - BSA and Azide free
アプリケーション適用あり: ICC/IF, Flow Cyt, WBmore details
適用なし: IHC-P or IP
Synthetic peptide within Human Nucleoside phosphorylase aa 200-300. The exact sequence is proprietary.
- WB: HeLa and Jurkat cell lysates.
ab247867 is the carrier-free version of ab109447 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247867 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 1.76 x 10 -10 M Learn more about KD
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247867 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 31 kDa (predicted molecular weight: 32 kDa).|
関連疾患Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
配列類似性Belongs to the PNP/MTAP phosphorylase family.
細胞内局在Cytoplasm > cytoskeleton.
- Information by UniProt
- FLJ94043 antibody
- FLJ97288 antibody
- FLJ97312 antibody
All lanes : Anti-Nucleoside phosphorylase antibody [EPR5715] (ab109447) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PNP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab109447).
ab109447 Anti-Nucleoside phosphorylase antibody [EPR5715] was shown to specifically react with Nucleoside phosphorylase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266158 (knockout cell lysate ab257594) was used. Wild-type and Nucleoside phosphorylase knockout samples were subjected to SDS-PAGE. ab109447 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.