Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5714] to Nucleoside phosphorylase - BSA and Azide free
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
製品名Anti-Nucleoside phosphorylase antibody [EPR5714] - BSA and Azide free
Nucleoside phosphorylase 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5714] to Nucleoside phosphorylase - BSA and Azide free
アプリケーション適用あり: IP, WBmore details
適用なし: Flow Cyt,ICC/IF or IHC-P
Synthetic peptide within Human Nucleoside phosphorylase (N terminal). The exact sequence is proprietary.
- WB: HeLa, Jurkat and JAR cell lysate.
ab247907 is the carrier-free version of ab109559 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab247907 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Concentration information loading...
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab247907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.|
関連疾患Defects in PNP are the cause of purine nucleoside phosphorylase deficiency (PNP deficiency) [MIM:613179]. It leads to a severe T-cell immunodeficiency with neurologic disorder in children.
配列類似性Belongs to the PNP/MTAP phosphorylase family.
細胞内局在Cytoplasm > cytoskeleton.
- Information by UniProt
- FLJ94043 antibody
- FLJ97288 antibody
- FLJ97312 antibody
All lanes : Anti-Nucleoside phosphorylase antibody [EPR5714] (ab109559) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PNP knockout HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 32 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab109559).
ab109559 Anti-Nucleoside phosphorylase antibody [EPR5714] was shown to specifically react with Nucleoside phosphorylase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab266158 (knockout cell lysate ab257594) was used. Wild-type and Nucleoside phosphorylase knockout samples were subjected to SDS-PAGE. ab109559 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.