Anti-Nucleoporin p62/NUP62 抗体 [2A] (ab188413)
Key features and details
- Rat monoclonal [2A] to Nucleoporin p62/NUP62
- Suitable for: IP, WB, ICC/IF
- Reacts with: Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-Nucleoporin p62/NUP62 antibody [2A]
Nucleoporin p62/NUP62 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [2A] to Nucleoporin p62/NUP62 -
由来種
Rat -
アプリケーション
適用あり: IP, WB, ICC/IFmore details -
種交差性
交差種: Human, African green monkey
非交差種: Mouse -
免疫原
Recombinant fragment (His-tag) corresponding to Human Nucleoporin p62/NUP62 aa 1-300. (N terminal proprietary tag).
Database link: P37198 -
エピトープ
aa 1-179 (FG-repeat region) -
ポジティブ・コントロール
- HeLa cells and Cos cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS -
Concentration information loading...
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精製度
Proprietary Purification -
特記事項(精製)
ab188413 was purified from serum-free culture medium of the hybridoma. -
ポリ/モノ
モノクローナル -
クローン名
2A -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab188413の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
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ICC/IF |
1/400.
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). |
ICC/IF
1/400. |
ターゲット情報
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関連性
The nuclear pore complex is a structure that extends across the nuclear envelope and regulates the flow of macromolecules between the cytoplasm and the nucleus. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. Nup 62 is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. There are multiple transcript variants of this gene, however, they encode a single protein isoform. This protein undergoes post-translational modifications. It contains about 10 N-acetylglucosamine side chain sites. -
細胞内局在
Nucleus; nuclear pore complex. Cytoplasm; cytoskeleton; spindle pole. Note: Central region of the nuclear pore, within the transporter. During mitotic cell division, it associates with the poles of the mitotic spindle. -
参照データベース
- Entrez Gene: 23636 Human
- Omim: 605815 Human
- SwissProt: P37198 Human
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別名
- 62 kDa nucleoporin antibody
- DKFZp547L134 antibody
- FLJ20822 antibody
see all
画像
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Anti-Nucleoporin p62/NUP62 antibody [2A] (ab188413) at 1/500 dilution + HeLa nuclear membrane lysate
Secondary
Alkaline phosphatase-conjugated anti-rat IgG
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunofluorescent analysis of HeLa cells labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100. -
Immunofluorescent analysis of HeLa cells, labeling Nucleoporin p62/NUP62 with ab188413 at 1/400 dilution, followed by Alexa Fluor®488-conjugated goat anti-rat IgG secondary antibody at 1/500 dilution. HeLa cells were fixed with 3.7% formaldehyde and
permeabilized with 0.5% Triton X-100.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab188413 は 6 報の論文で使用されています。
- Gleixner AM et al. NUP62 localizes to ALS/FTLD pathological assemblies and contributes to TDP-43 insolubility. Nat Commun 13:3380 (2022). PubMed: 35697676
- Lin YC et al. Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway. Nat Neurosci 24:1077-1088 (2021). PubMed: 34059832
- Heusel M et al. A Global Screen for Assembly State Changes of the Mitotic Proteome by SEC-SWATH-MS. Cell Syst 10:133-155.e6 (2020). PubMed: 32027860
- Fišerová J et al. Nuclear pore protein TPR associates with lamin B1 and affects nuclear lamina organization and nuclear pore distribution. Cell Mol Life Sci 76:2199-2216 (2019). PubMed: 30762072
- Fišerová J et al. Chromatin organization at the nuclear periphery as revealed by image analysis of structured illumination microscopy data. J Cell Sci 130:2066-2077 (2017). PubMed: 28476938
- Linder MI et al. Mitotic Disassembly of Nuclear Pore Complexes Involves CDK1- and PLK1-Mediated Phosphorylation of Key Interconnecting Nucleoporins. Dev Cell 43:141-156.e7 (2017). PubMed: 29065306