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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-Nuclear Receptor Corepressor NCoR 抗体 (ab3482)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (9)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

Key features and details

  • Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-Nuclear Receptor Corepressor NCoR antibody
    Nuclear Receptor Corepressor NCoR 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443.
    Sequence:

    PAPLLSAQYETLSDSDD


    (Peptide available as ab4997)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Nuclear Receptor Corepressor NCoR protein (ab82239)

アプリケーション

Our Abpromise guarantee covers the use of ab3482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/1000.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能

    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • 配列類似性

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • ドメイン

    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • 翻訳後修飾

    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession O75376 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 9611 Human
    • Entrez Gene: 20185 Mouse
    • Entrez Gene: 54299 Rat
    • Entrez Gene: 398304 Xenopus laevis
    • Omim: 600849 Human
    • SwissProt: O75376 Human
    • SwissProt: Q60974 Mouse
    • SwissProt: Q9WUB5 Rat
    • SwissProt: Q8QG78 Xenopus laevis
    • Unigene: 462323 Human
    • Unigene: 271814 Mouse
    • Unigene: 460227 Mouse
    • Unigene: 24948 Rat
    • Unigene: 77498 Xenopus laevis
    see all
  • 別名

    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (9)

    ab3482 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab3482 は 9 報の論文で使用されています。

    • Jeon YG  et al. RNF20 Functions as a Transcriptional Coactivator for PPAR? by Promoting NCoR1 Degradation in Adipocytes. Diabetes 69:20-34 (2020). PubMed: 31604693
    • Zylicz JJ  et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell 176:182-197.e23 (2019). PubMed: 30595450
    • Raciti M  et al. NRXN1 Deletion and Exposure to Methylmercury Increase Astrocyte Differentiation by Different Notch-Dependent Transcriptional Mechanisms. Front Genet 10:593 (2019). PubMed: 31316548
    • Balasubramaniyan N  et al. Nuclear factor-?B regulates the expression of multiple genes encoding liver transport proteins. Am J Physiol Gastrointest Liver Physiol 310:G618-28 (2016). PubMed: 26867564
    • Kim H The transcription cofactor CRTC1 protects from aberrant hepatic lipid accumulation. Sci Rep 6:37280 (2016). WB ; Mouse . PubMed: 27869139
    • Liu YY  et al. Thyroid hormone receptor sumoylation is required for preadipocyte differentiation and proliferation. J Biol Chem 290:7402-15 (2015). WB ; Mouse . PubMed: 25572392
    • Corbett GT  et al. Activation of peroxisome proliferator-activated receptor a stimulates ADAM10-mediated proteolysis of APP. Proc Natl Acad Sci U S A 112:8445-50 (2015). ChIP ; Mouse . PubMed: 26080426
    • Tsuji-Takechi K  et al. Stage-specific functions of leukemia/lymphoma-related factor (LRF) in the transcriptional control of osteoclast development. Proc Natl Acad Sci U S A 109:2561-6 (2012). ChIP ; Mouse . PubMed: 22308398
    • You SH  et al. 4-Hydroxy-PCB106 acts as a direct thyroid hormone receptor agonist in rat GH3 cells. Mol Cell Endocrinol 257-258:26-34 (2006). PubMed: 16930818

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    1-5 of 5 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (skin)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 30°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Sep 22 2015

    Western blot abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HEK293)
    Loading amount
    20 µg
    Specification
    HEK293
    Gel Running Conditions
    Non-reduced Denaturing (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 01 2013

    Question

    Hi,
    I would like to know if it's possible to buy a larger Qty of NCOR1 abcam #3482 antibody. We use this antibody a lot in the laboratory and it's would be nice to have a larger Qty or a special price if we buy a couple of tubes of this antobody.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 24 2012

    Answer

    Thank you for contacting Abcam.

    We do not have any other sizes of ab3482 in stock. If you order 5 or more vials, we can offer a 5% discount and if you wish to place an order for 10 or more vials, I can out you in contact with our bulk sales team and they can work out a good deal for you.

    If there is anything else I can help you with, or if you are interested in placing a bulk (>10vials) order, then please let me know.

    Read More

    Abcam Scientific Support

    Answered on Oct 24 2012

    Question

    What is recommended to use a positive control for Western blotting with this antibody?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 26 2005

    Answer

    Thank you for your enquiry. Ab3482 was characterized in Western blotting using HeLa cell extract; by Western blot, this antibody detects a protein representing N-CoR from HeLa cell extracts. HeLa cell extract is therefore recommended as a positive control for this antibody. This antibody has so far been tested for cross-reactivity with human and has not yet been tested in other species. The datasheet previously said mouse, but I have updated it to reflect this new information that I obtained from the antibody's originator. If you have any additional questions, please let us know. We are still working on your question regarding ab84 and will get back to you soon.

    Read More

    Abcam Scientific Support

    Answered on Jul 27 2005

    Question

    I was wondering if you could tell me whether your polyclonal rabbit Ncor1 antibody binds to Ncor's DNA-binding domain or not? We plan to use it for gel-shift analysis, so it is important that it does not.

    Read More

    Abcam community

    Verified customer

    Asked on May 24 2004

    Answer

    Thank you for your enquiry and your interest in our product. We have not tested this yet. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

    Read More

    Abcam Scientific Support

    Answered on May 26 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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