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    nuclear-receptor-corepressor-ncor-antibody-ab3482.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
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Anti-Nuclear Receptor Corepressor NCoR 抗体 (ab3482)

  • Datasheet
  • SDS
Reviews (2)Q&A (3)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

Key features and details

  • Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • Suitable for: ChIP, WB, IP, IHC-P, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-Nuclear Receptor Corepressor NCoR antibody
    Nuclear Receptor Corepressor NCoR 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Nuclear Receptor Corepressor NCoR
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ChIP, WB, IP, IHC-P, ICC/IFmore details
  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat, Xenopus laevis, Xenopus tropicalis
  • 免疫原

    Synthetic peptide corresponding to Mouse Nuclear Receptor Corepressor NCoR aa 2427-2443.
    Sequence:

    PAPLLSAQYETLSDSDD


    (Peptide available as ab4997)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Coactivators and repressors
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Nuclear Receptor Corepressor NCoR protein (ab82239)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3482の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ChIP
Use at an assay dependent concentration.

Pubmed ID: 16930818.

WB (1)
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.

By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages.

IP
Use at an assay dependent concentration.
IHC-P (1)
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.
特記事項
ChIP
Use at an assay dependent concentration.

Pubmed ID: 16930818.

WB
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.

By Western blot, this antibody detects a protein at ~270 kDa representing N-CoR from HeLa cell extracts and mouse RAW 264.7/primary macrophages.

IP
Use at an assay dependent concentration.
IHC-P
1/200 - 1/1000.
ICC/IF
1/100 - 1/500.

ターゲット情報

  • 機能

    Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.
  • 配列類似性

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • ドメイン

    The N-terminal region contains three independent domains that are capable of mediating transcriptional repression (RD1, RD2 and RD3).
    The C-terminal region contains two separate nuclear receptor-interacting domains (ID1 and ID2), each of which contains a conserved sequence referred to as the CORNR box. This motif is necessary and sufficient for binding to unligated nuclear hormone receptors, while sequences flanking the CORNR box determine the precise nuclear hormone receptor specificity.
  • 翻訳後修飾

    Ubiquitinated; mediated by SIAH2 and leading to its subsequent proteasomal degradation.
  • 細胞内局在

    Nucleus.
  • Target information above from: UniProt accession O75376 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 9611 Human
    • Entrez Gene: 20185 Mouse
    • Entrez Gene: 54299 Rat
    • Entrez Gene: 398304 Xenopus laevis
    • Omim: 600849 Human
    • SwissProt: O75376 Human
    • SwissProt: Q60974 Mouse
    • SwissProt: Q9WUB5 Rat
    • SwissProt: Q8QG78 Xenopus laevis
    • Unigene: 462323 Human
    • Unigene: 271814 Mouse
    • Unigene: 460227 Mouse
    • Unigene: 24948 Rat
    • Unigene: 77498 Xenopus laevis
    see all
  • 別名

    • hN CoR antibody
    • hNCoR antibody
    • KIAA1047 antibody
    • N CoR antibody
    • N Cor/SMRT corepressor Rip13 antibody
    • N CoR1 antibody
    • N-CoR antibody
    • N-CoR1 antibody
    • NCOR 1 antibody
    • NCoR antibody
    • Ncor1 antibody
    • NCOR1_HUMAN antibody
    • Nuclear receptor co repressor 1 antibody
    • Nuclear receptor corepressor 1 antibody
    • Retinoid X receptor interacting protein 13 antibody
    • RIP13 antibody
    • Rxrip13 antibody
    • thyroid hormone and retinoic acid receptor associated corepressor 1 antibody
    • TRAC 1 antibody
    • TRAC1 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Mouse breast tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR in the nucleus of Human breast carcinoma (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Tissue was blocked in 3% H2O2-methanol for 15 min at room temperature, then incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)
    Immunocytochemistry/ Immunofluorescence - Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade (ab3482)

    ab3482 labelling Nuclear Receptor Corepressor NCoR (green) in the nucleus and cytoplasm of HeLa cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:200 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (12)

ab3482 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3482 は 12 報の論文で使用されています。

  • Wang Y  et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
  • Garg M  et al. Cardiolipin-mediated PPAR? S112 phosphorylation impairs IL-10 production and inflammation resolution during bacterial pneumonia. Cell Rep 34:108736 (2021). PubMed: 33567272
  • Moretti C  et al. Battle of the Sex Chromosomes: Competition between X and Y Chromosome-Encoded Proteins for Partner Interaction and Chromatin Occupancy Drives Multicopy Gene Expression and Evolution in Muroid Rodents. Mol Biol Evol 37:3453-3468 (2020). PubMed: 32658962
  • Jeon YG  et al. RNF20 Functions as a Transcriptional Coactivator for PPAR? by Promoting NCoR1 Degradation in Adipocytes. Diabetes 69:20-34 (2020). PubMed: 31604693
  • Zylicz JJ  et al. The Implication of Early Chromatin Changes in X Chromosome Inactivation. Cell 176:182-197.e23 (2019). PubMed: 30595450
View all Publications for this product

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1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer ph6 20 min at 97ºC IN A PT LINK
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 30°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 22 2015

Western blot abreview for Anti-Nuclear Receptor Corepressor NCoR antibody - ChIP Grade

Good
Abreviews
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Gel Running Conditions
Non-reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 01 2013

Question

Hi,
I would like to know if it's possible to buy a larger Qty of NCOR1 abcam #3482 antibody. We use this antibody a lot in the laboratory and it's would be nice to have a larger Qty or a special price if we buy a couple of tubes of this antobody.

Read More

Abcam community

Verified customer

Asked on Oct 24 2012

Answer

Thank you for contacting Abcam.

We do not have any other sizes of ab3482 in stock. If you order 5 or more vials, we can offer a 5% discount and if you wish to place an order for 10 or more vials, I can out you in contact with our bulk sales team and they can work out a good deal for you.

If there is anything else I can help you with, or if you are interested in placing a bulk (>10vials) order, then please let me know.

Read More

Abcam Scientific Support

Answered on Oct 24 2012

Question

What is recommended to use a positive control for Western blotting with this antibody?

Read More

Abcam community

Verified customer

Asked on Jul 26 2005

Answer

Thank you for your enquiry. Ab3482 was characterized in Western blotting using HeLa cell extract; by Western blot, this antibody detects a protein representing N-CoR from HeLa cell extracts. HeLa cell extract is therefore recommended as a positive control for this antibody. This antibody has so far been tested for cross-reactivity with human and has not yet been tested in other species. The datasheet previously said mouse, but I have updated it to reflect this new information that I obtained from the antibody's originator. If you have any additional questions, please let us know. We are still working on your question regarding ab84 and will get back to you soon.

Read More

Abcam Scientific Support

Answered on Jul 27 2005

Question

I was wondering if you could tell me whether your polyclonal rabbit Ncor1 antibody binds to Ncor's DNA-binding domain or not? We plan to use it for gel-shift analysis, so it is important that it does not.

Read More

Abcam community

Verified customer

Asked on May 24 2004

Answer

Thank you for your enquiry and your interest in our product. We have not tested this yet. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

Read More

Abcam Scientific Support

Answered on May 26 2004

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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