Anti-Nuclear Pore O-Linked Glycoprotein 抗体 [RL1] (ab2734)

Submit an Abreview Q&A (2)Specific References (7)

製品の概要

  • 製品名
    Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1]
  • 製品の詳細
    Mouse monoclonal [RL1] to Nuclear Pore O-Linked Glycoprotein
  • 由来種
    Mouse
  • 特異性
    Detects nuclear pore-O-linked glycoprotein
  • アプリケーション
    適用あり: IHC-P, Blocking, ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human, Saccharomyces cerevisiae, Xenopus laevis, Amphibian
    交差が予測される動物種: Mammals
  • 免疫原

    Other Immunogen Type corresponding to Rat Nuclear Pore O-Linked Glycoprotein. Pore complex-lamina fraction purified from rat liver nuclear envelopes.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.
ICC/IF 1/100.
IP Use at an assay dependent concentration.
WB 1/1000.

ターゲット情報

  • 関連性
    Diffusion of metabolites and small non-nuclear molecules as well as active, mediated import of protein and export of protein and RNA through the nuclear envelope occurs through nuclear pore complexes or NPC’s. NPC’s contain up to 100 different polypeptides which have a combined mass of about 125 megadaltons. The channel available for passive transport through the NPC is about 9-10 nm in diameter while carrier mediated changes in the NPC result in a ~25 nm channel used for larger, actively transported molecules. Of the 100 polypeptides, at least 8 of these are O-linked N-acetylglycosamine-modified in mammalian cells. All of the mammalian O-linked glycoproteins contain multiple copies of phenylalanine, glycine dipeptide repeats dispersed throughout part of their sequence. Studies indicate that the NPC O-linked glycoproteins have a direct role in nuclear protein import.
  • 細胞内局在
    Nuclear membrane

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat lymph node tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nuclear Pore O-Linked Glycoprotein antibody [RL1] (ab2734)
    Immunohistochemistry was performed on normal biopsies of deparaffinized Rat kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Nuclear Pore-O-Linked Glycoprotein ab2734 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

参考文献

This product has been referenced in:
  • Gorsch LC  et al. A conditional allele of the novel repeat-containing yeast nucleoporin RAT7/NUP159 causes both rapid cessation of mRNA export and reversible clustering of nuclear pore complexes. J Cell Biol 129:939-55 (1995). Read more (PubMed: 7744966) »
  • Guan T  et al. Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol Biol Cell 6:1591-603 (1995). Read more (PubMed: 8589458) »

See all 7 Publications for this product

ab2734 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

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I was just wondering if you know what nuclear pore proteins this antibody is detecting in yeast.

Thank you for your enquiry. I can confirm that this product detects nuclear pore-O-linked glycoprotein from rat, amphibian and yeast. I hope this information will be useful to you. If you require any further information, please do not hesitate to...

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Which NPC proteins does this antibody detect? I am also seeing a 250kDa band on my Western blot. Has this band been observed before?

Thank you for your enquiry. I am unaware of any resources that mention a nuclear pore protein at 250 kDa. I did a quick literature search and did not pull up a nuclear pore protein at 250 kDa. However, I did find an interesting article that states...

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