Anti-NTAL 抗体 [NAP-07] (ab3992)

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Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)

    Key features and details

    • Mouse monoclonal [NAP-07] to NTAL
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG1

    Conjugates logo Related conjugates and formulations

    PE

    製品の概要

    • 製品名

      Anti-NTAL antibody [NAP-07]
      NTAL 一次抗体 製品一覧

    • 製品の詳細

      Mouse monoclonal [NAP-07] to NTAL

    • 由来種

      Mouse

    • 特異性

      The antibody reacts with Non-T cell activation linker (NTAL) a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.

    • アプリケーション

      適用あり: ICC/IFmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Recombinant fragment, corresponding to amino acids 91-244 of Human NTAL.

    • ポジティブ･コントロール

      • RAMOS human lymphoma cell lysate, Raji human lymphoma cell lysate

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab3992の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    ICC/IF
    Use at an assay dependent concentration.
    特記事項
    ICC/IF
    Use at an assay dependent concentration.

    ターゲット情報

    • 機能

      Involved in FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. May also be involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and FCGR1 (high affinity immunoglobulin gamma Fc receptor I)-mediated signaling in myeloid cells. Couples activation of these receptors and their associated kinases with distal intracellular events through the recruitment of GRB2.

    • 組織特異性

      Highly expressed in spleen, peripheral blood lymphocytes, and germinal centers of lymph nodes. Also expressed in placenta, lung, pancreas and small intestine. Present in B-cells, NK cells and monocytes. Absent from T-cells (at protein level).

    • 関連疾患

      Note=LAT2 is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of LAT2 may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease.

    • 翻訳後修飾

      Phosphorylated on tyrosines following cross-linking of BCR in B-cells, FCGR1 in myeloid cells, or FCER1 in mast cells; which induces the recruitment of GRB2.
      May be polyubiquitinated.

    • 細胞内局在

      Cell membrane. Present in lipid rafts.
    • Information by UniProt

    • 参照データベース

      • 別名

        • HSPC046 antibody
        • LAB antibody
        • LAT2 antibody
        • Linker for activation of B cells antibody
        • Linker for activation of B-cells antibody
        • Linker for activation of T cells family member 2 antibody
        • Linker for activation of T cells transmembrane adaptor 2 antibody
        • Linker for activation of T-cells family member 2 antibody
        • Membrane associated adapter molecule antibody
        • Membrane-associated adapter molecule antibody
        • Non T cell activation linker antibody
        • Non-T-cell activation linker antibody
        • NTAL_HUMAN antibody
        • WBSCR 5 antibody
        • Wbscr15 antibody
        • Williams Beuren syndrome chromosome region 15 antibody
        • Williams Beuren syndrome chromosome region 5 antibody
        • Williams-Beuren syndrome chromosomal region 15 protein antibody
        • Williams-Beuren syndrome chromosomal region 5 protein antibody
        • WSCR5 antibody
        see all

      画像

      • Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Immunocytochemistry/ Immunofluorescence - Anti-NTAL antibody [NAP-07] (ab3992)
        Subcellular localization of NTAL by confocal microscopy in the myeloid cell line THP-1. THP-1 cells were permeabilized and immunostained using antibody NAP-07 (green). Nuclei are vizualized by propidium iodide (red).

      参考文献 (8)

      ab3992 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab3992 は 8 報の論文で使用されています。

      • Malhotra S  et al. Vav and Rac activation in B cell antigen receptor endocytosis involves Vav recruitment to the adapter protein LAB. J Biol Chem 284:36202-12 (2009). WB ; Mouse . PubMed: 19858206
      • Mutch CM  et al. Activation-induced endocytosis of the raft-associated transmembrane adaptor protein LAB/NTAL in B lymphocytes: evidence for a role in internalization of the B cell receptor. Int Immunol 19:19-30 (2007). PubMed: 17090619
      • Macaulay IC  et al. Comparative gene expression profiling of in vitro differentiated megakaryocytes and erythroblasts identifies novel activatory and inhibitory platelet membrane proteins. Blood 109:3260-9 (2007). PubMed: 17192395
      • Dráberová L  et al. Regulation of Ca2+ signaling in mast cells by tyrosine-phosphorylated and unphosphorylated non-T cell activation linker. J Immunol 179:5169-80 (2007). PubMed: 17911602
      • Lebduska P  et al. Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells. J Immunol Methods 328:139-51 (2007). PubMed: 17900607
      • Heneberg P  et al. Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol 36:2795-806 (2006). PubMed: 17013982
      • Volná P  et al. Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL. J Exp Med 200:1001-13 (2004). PubMed: 15477348
      • Tkaczyk C  et al. NTAL phosphorylation is a pivotal link between the signaling cascades leading to human mast cell degranulation following Kit activation and Fc epsilon RI aggregation. Blood 104:207-14 (2004). PubMed: 15010370

      レビューと Q&A

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      Western blot abreview for Anti-NTAL antibody [NAP-07]

       Excellent
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Cell lysate - other (RAW264.7)
      Loading amount
      40 µg
      Specification
      RAW264.7
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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      投稿 Oct 24 2007

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