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Anti-NT-proBNP 抗体 [15F11] (ab13115)

Submit a review Q&A (1)References (5)
Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
  • ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)

Key features and details

  • Mouse monoclonal [15F11] to NT-proBNP
  • Suitable for: ELISA, WB
  • Reacts with: Recombinant fragment
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-NT-proBNP antibody [15F11]
    NT-proBNP 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [15F11] to NT-proBNP

  • 由来種

    Mouse

  • アプリケーション

    適用あり: ELISA, WBmore details

  • 種交差性

    交差種: Recombinant fragment

  • 免疫原

    Synthetic peptide corresponding to Human NT-proBNP aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Recombinant Human BNP protein (ab87200) can be used as a positive control in WB.

  • 特記事項

    Immunoglobulin concentrations vary from batch to batch. Please contact technical services for the latest information.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab13115の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.
特記事項
ELISA
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 13 kDa.

ターゲット情報

  • 機能

    Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3.

  • 組織特異性

    Brain and also in atria, but at much lower levels than ANP.

  • 配列類似性

    Belongs to the natriuretic peptide family.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 別名

    • ANFB_HUMAN antibody
    • BNP(1-32) antibody
    • BNP(5-29) antibody
    • BNP-32 antibody
    • Gamma-brain natriuretic peptide antibody
    • nppb antibody
    see all

画像

  • Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
    Western blot - Anti-NT-proBNP antibody [15F11] (ab13115)
    WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11]. WB detecting proBNP showing comparable detection from a range of antibodies available. Lane 1 - ab13111 [5B6], lane 2 - ab13115 [15F11], lane 3 - ab13059 [11D1], lane 4 - ab13124 [15D7], lane 5 - ab13122 [13C1], lane 6 ab13123 [24E11].
  • ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)
    ELISA - Anti-NT-proBNP antibody [15F11] (ab13115)
    NT-proBNP calibration curves using pairs ab14703 [10H4] and ab13111 [5B6] (grey), and ab13115 [15F11] and ab13111 [5B6] (black), (capture: 2 µg/well, detection: Eu-labeled 5B6; 200 ng/well). Antigen was recombinant NT-proBNP. Incubated 1 hr at room temperature

参考文献 (5)

ab13115 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab13115 は 5 報の論文で使用されています。

  • Karhu ST  et al. GATA4-targeted compound exhibits cardioprotective actions against doxorubicin-induced toxicity in vitro and in vivo: establishment of a chronic cardiotoxicity model using human iPSC-derived cardiomyocytes. Arch Toxicol 94:2113-2130 (2020). PubMed: 32185414
  • Pohjolainen L  et al. Pharmacological Protein Kinase C Modulators Reveal a Pro-hypertrophic Role for Novel Protein Kinase C Isoforms in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Pharmacol 11:553852 (2020). PubMed: 33584253
  • Mosqueira D  et al. High-Throughput Phenotyping Toolkit for Characterizing Cellular Models of Hypertrophic Cardiomyopathy In Vitro. Methods Protoc 2:N/A (2019). PubMed: 31717790
  • Nagai H  et al. Antihypertrophic Effects of Small Molecules that Maintain Mitochondrial ATP Levels Under Hypoxia. EBioMedicine 24:147-158 (2017). PubMed: 28942281
  • Tonne JM  et al. Secretion of glycosylated pro-B-type natriuretic peptide from normal cardiomyocytes. Clin Chem 57:864-73 (2011). IP ; Human . PubMed: 21482747

レビューと Q&A

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Question

Inquiry: Dear Sir/Madam, I am looking for antibodies directed at or cross-reactive with rat NT-pro-BNP. Preferably monoclonal and source from any species. There are multiple monoclonal available on your site, but directed to human. Do you have antibodies that are crossreactive to rat. Best Regards,

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Answer

Thank you very much for your inquiry.

I have to confirm that do not have any pro-BNP antibodies that are tested and guaranteed for rat.

I checked the immunogens for the available pro-BNP antibodies and unfortunately I cannot recommend any of for testing in rat.

We usually expect an cross-reaction when the homology is 85% and higher. But the different regions that the immunogens are taken from only show 62% and less homology:

AA 1-12 no homology
AA 13-27 58%
AA 28-45 62%
AA 61-76 no homology.

I am sorry I could not be of more help on thisoccasion and wish you goo dluck with your research.

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