Anti-NSUN2/SAKI 抗体 (ab272624)
Key features and details
- Rabbit polyclonal to NSUN2/SAKI
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NSUN2/SAKI antibody
NSUN2/SAKI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NSUN2/SAKI -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Recombinant fragment corresponding to Human NSUN2/SAKI aa 500-650.
Database link: Q08J23 -
ポジティブ・コントロール
- WB: U-2 OS cell lysate. IHC-P: Human duodenum tissue, testis tissue, skin tissue, skeletal tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab272624の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49. May act downstream of Myc to regulate epidermal cell growth and proliferation. -
配列類似性
Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily. -
翻訳後修飾
Phosphorylated at Ser-139 by Aurora-B/STK12 during mitosis, leading to abolish methyltransferase activity and the interaction with NPM1. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Concentrated in the nucleolus during interphase and distributed in the perichromosome and cytoplasm during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 54888 Human
- Entrez Gene: 28114 Mouse
- Omim: 610916 Human
- SwissProt: Q08J23 Human
- SwissProt: Q1HFZ0 Mouse
- Unigene: 481526 Human
- Unigene: 260009 Mouse
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別名
- 5 methycytoisine methyltransferase antibody
- D13Wsu123e antibody
- FLJ20303 antibody
see all
画像
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Lanes 1 & 3 : Anti-NSUN2/SAKI antibody (ab272624) at 0.4 µg/ml
Lane 2 : Anti-NSUN2/SAKI antibody (ab272624)
Lane 1 : U-2 OS cells transfected with target siRNA 1
Lane 2 : U-2 OS cells transfected with target siRNA 2
Lane 3 : U-2 OS cells transfected with control siRNA -
Paraffin-embedded formalin-fixed Human duodenum tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.
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Paraffin-embedded formalin-fixed Human testis tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.
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Paraffin-embedded formalin-fixed Human skin tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.
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Paraffin-embedded formalin-fixed Human skeletal tissue stained for NSUN2/SAKI using ab272624 at 1/1000 in immunohistochemical analysis.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab272624 は 1 報の論文で使用されています。
- Sun Z et al. Aberrant NSUN2-mediated m5C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma. Oncogene 39:6906-6919 (2020). PubMed: 32978516