Anti-NSE 抗体 - Neuronal Marker (ab834)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)

    Key features and details

    • Rabbit polyclonal to NSE - Neuronal Marker
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-NSE antibody - Neuronal Marker
      NSE 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to NSE - Neuronal Marker

    • 由来種

      Rabbit

    • 特異性

      This antibody reacts with human isoenzymes of neuron specific enolase containing gamma subunits. This antibody gives a specific staining in neurons of the brain, in islets of langerhans in pancreas and in peripheral nerves of the skin. No staining with other tissue types has been seen.

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Neuron specific enolase (gamma-gamma isoenzyme) isolated from bovine brain.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab834の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    特記事項
  • 追加情報
    IHC: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method.
    Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user.

    • ターゲット情報

    • 機能

      Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival.

    • 組織特異性

      The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

    • パスウェイ

      Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.

    • 配列類似性

      Belongs to the enolase family.

    • 発生段階

      During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.

    • 細胞内局在

      Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.
    • Information by UniProt

    • 参照データベース

      • 別名

        • 2 phospho D glycerate hydrolyase antibody
        • 2-phospho-D-glycerate hydro-lyase antibody
        • Eno 2 antibody
        • ENO2 antibody
        • ENOG antibody
        • ENOG_HUMAN antibody
        • Enolase 2 (gamma, neuronal) antibody
        • Enolase 2 antibody
        • Enolase 2 gamma neuronal antibody
        • Enolase2 antibody
        • Epididymis secretory protein Li 279 antibody
        • Gamma enolase antibody
        • Gamma-enolase antibody
        • HEL S 279 antibody
        • Neural enolase antibody
        • Neuron specific enolase antibody
        • Neuron specific gamma enolase antibody
        • Neuron-specific enolase antibody
        • neuronal enriched enolase antibody
        • Neurone specific enolase antibody
        • NSE antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NSE antibody - Neuronal Marker (ab834)
        Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue, staining NSE - Neuronal Marker with ab834.

      参考文献 (1)

      ab834 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab834 は 1 報の論文で使用されています。

      • Tang YY  et al. Ginsenoside Rg1 Promotes the Migration of Olfactory Ensheathing Cells via the PI3K/Akt Pathway to Repair Rat Spinal Cord Injury. Biol Pharm Bull 40:1630-1637 (2017). PubMed: 28966235

      レビューと Q&A

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      Question

      Is NSE sensitive to triton-X? Should I be leaving this out of my IHC protocol, or can I leave it in?

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      Answer

      We recommend the use of 0.025% Triton in the TBS as it helps to reduce surface tension, allowing reagents to cover the whole tissue section with ease. It is also believed to dissolve Fc receptors, therefore reducing non-specific binding. Furthermore, TBS gives a cleaner background than PBS. Care must be taken using Triton when working with membrane-bound proteins as the Triton acts as a surfactant and strips the lipid component out of the membrane. Neuron specific enolase (NSE) is located in the cytoplasm of mature neurons so you should have no problem using Triton in your buffer.

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