Anti-NSE 抗体 - Neuronal Marker (ab53025)
Key features and details
- Rabbit polyclonal to NSE - Neuronal Marker
- Suitable for: ELISA, ICC/IF, WB, IHC-P, IHC-FoFr, Flow Cyt
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-NSE antibody - Neuronal Marker
NSE 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NSE - Neuronal Marker -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, ICC/IF, WB, IHC-P, IHC-FoFr, Flow Cytmore details -
種交差性
交差種: Mouse, Rat, Human, Pig -
免疫原
Synthetic peptide derived from human NSE
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53025の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use a concentration of 1 - 5 µg/ml.
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WB | (3) |
1/500 - 1/1000. Predicted molecular weight: 47 kDa.
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IHC-P | (5) |
Use at an assay dependent concentration.
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IHC-FoFr | (1) |
Use at an assay dependent concentration.
|
Flow Cyt | (1) |
Use at an assay dependent concentration.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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ELISA
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
WB
1/500 - 1/1000. Predicted molecular weight: 47 kDa. |
IHC-P
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival. -
組織特異性
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5. -
配列類似性
Belongs to the enolase family. -
発生段階
During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. -
細胞内局在
Cytoplasm. Cell membrane. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. - Information by UniProt
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参照データベース
- Entrez Gene: 2026 Human
- Entrez Gene: 13807 Mouse
- Entrez Gene: 24334 Rat
- Omim: 131360 Human
- SwissProt: P09104 Human
- SwissProt: P17183 Mouse
- SwissProt: P07323 Rat
- Unigene: 511915 Human
see all -
別名
- 2 phospho D glycerate hydrolyase antibody
- 2-phospho-D-glycerate hydro-lyase antibody
- Eno 2 antibody
see all
画像
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All lanes : Anti-NSE antibody - Neuronal Marker (ab53025) at 1/500 dilution
Lane 1 : Extracts from HepG2 cells with no immunizing peptide
Lane 2 : Extracts from HepG2 cells with immunizing peptide
Predicted band size: 47 kDa
Observed band size: 47 kDa -
ICC/IF image of ab53025 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53025, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemical analysis of paraffin-embedded human brain tissue using ab53025 at 1/50-1/100 dilution. Left: without immunizing peptide; Right: with immunizing peptide
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ab53025 staining NSE in mouse brain tissue section by Immunohistochemistry (PFA-perfussion fixed frozen tissue sections). Tissues were collected from mice in each group at 10 weeks. Mice were euthanized, the brains were exposed and removed from the body. Then the brain was cut, on ice and fixed in 4% polyformaldehyde or glutaraldehyde solution for histological examination. The primary antibody was used at 1/75 dilution in PBS and incubated with sample for 2 hours at 37°C. A HRP-conjugated secondary antibody was used at for 1hr at 37°C in a humidity box.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (58)
ab53025 は 58 報の論文で使用されています。
- Jacobson JC et al. Combination bromo- and extraterminal domain and poly (ADP-ribose) polymerase inhibition synergistically enhances DNA damage and inhibits neuroblastoma tumorigenesis. Discov Oncol 13:103 (2022). PubMed: 36227363
- Sun C et al. Overexpression of enolase 2 is associated with worsened prognosis and increased glycikolysis in papillary renal cell carcinoma. J Cell Physiol 236:3821-3831 (2021). PubMed: 33135164
- Dutta DJ & Fields RD Deletion of the Thrombin Proteolytic Site in Neurofascin 155 Causes Disruption of Nodal and Paranodal Organization. Front Cell Neurosci 15:576609 (2021). PubMed: 33815060
- Yamagiwa Y et al. Orbital malignant schwannoma and embryonal rhabdomyosarcoma in rats caused by leakage of locally injected nickel subsulfide to the orbit. J Toxicol Pathol 34:151-156 (2021). PubMed: 33976471
- Chen J et al. Evaluation of the Effectiveness of a Chronic Ocular Hypertension Mouse Model Induced by Intracameral Injection of Cross-Linking Hydrogel. Front Med (Lausanne) 8:643402 (2021). PubMed: 33829024