Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Treatment
cigarette smoke for 0.5-24 hrs
Specification
ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: ECL Rabbit IgG, HRP-linked whole Ab from donkey
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Donkey
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 80 kDa
Positive control
CSE treated cell lysates
Additional data
Additional Notes
figure legend
1 – DMSO 24h
2 – CSE 24h
3 – DMSO 12h
4 – CSE 12h
5 – DMSO 6h
6 – CSE 6h
7 – DMSO 3h
8 – CSE 3h
9 – DMSO 1h
10 – CSE 1h
11 – DMSO 0.5h
12 – CSE 0.5h
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lei Wang
Verified customer
投稿 Jul 07 2017